Force-dependent integrin-cytoskeleton linkage formation requires downregulation of focal complex dynamics by Shp2

Götz Von Wichert, Beatrice Haimovich, Gen Sheng Feng, Michael Sheetz

Research output: Contribution to journalArticle

169 Citations (Scopus)

Abstract

As cells encounter new regions of the substrate, they develop bonds with new matrix molecules for migration, matrix remodeling and force generation. How cells orchestrate the assembly of adhesion sites is only partially understood. Here we show that fibroblasts deficient in the SH2 domain containing protein tyrosine phosphatase 2 (Shp2) have an increased number of immature focal complexes deficient in α-actinin. Inhibition of FAK restored α-actinin to focal complexes, whereas inhibition of RhoA did not. In correlation, adhesion site dynamics, measured by fluorescence recovery after photobleaching (FRAP) of GFP-paxillin and GFP-vinculin were dramatically increased in Shp2-/- cells and restored to normal by FAK inhibition. Shp2-/- cells failed to strengthen initial integrin-cytoskeleton linkages, as measured by optical tweezers and large bead assays, and were rescued by inhibition of FAK. In contrast, affinity modulation of adhesion receptors was unaffected. In addition, reinforcement correlated with α-actinin assembly through decreased dynamics. This shows for the first time that adhesion site dynamics are regulated during adhesion formation and that force-dependent strengthening of integrin-cytoskeleton linkages is correlated with α-actinin assembly and decreased adhesion site dynamics.

Original languageEnglish (US)
Pages (from-to)5023-5035
Number of pages13
JournalEMBO Journal
Volume22
Issue number19
DOIs
StatePublished - Oct 1 2003
Externally publishedYes

Fingerprint

Actinin
Cytoskeleton
Integrins
Down-Regulation
Adhesion
SH2 Domain-Containing Protein Tyrosine Phosphatases
Optical Tweezers
Fluorescence Recovery After Photobleaching
Paxillin
Vinculin
Protein Phosphatase 2
Optical tweezers
Photobleaching
Fibroblasts
Assays
Reinforcement
Fluorescence
Modulation
Recovery
Molecules

Keywords

  • Focal adhesion kinase
  • Focal complex
  • Force
  • FRAP
  • Shp2

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Force-dependent integrin-cytoskeleton linkage formation requires downregulation of focal complex dynamics by Shp2. / Von Wichert, Götz; Haimovich, Beatrice; Feng, Gen Sheng; Sheetz, Michael.

In: EMBO Journal, Vol. 22, No. 19, 01.10.2003, p. 5023-5035.

Research output: Contribution to journalArticle

Von Wichert, Götz ; Haimovich, Beatrice ; Feng, Gen Sheng ; Sheetz, Michael. / Force-dependent integrin-cytoskeleton linkage formation requires downregulation of focal complex dynamics by Shp2. In: EMBO Journal. 2003 ; Vol. 22, No. 19. pp. 5023-5035.
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