Force-Induced Calpain Cleavage of Talin Is Critical for Growth, Adhesion Development, and Rigidity Sensing

Mayur Saxena, Rishita Changede, James Hone, Haguy Wolfenson, Michael P. Sheetz

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


Cell growth depends upon formation of cell-matrix adhesions, but mechanisms detailing the transmission of signals from adhesions to control proliferation are still lacking. Here, we find that the scaffold protein talin undergoes force-induced cleavage in early adhesions to produce the talin rod fragment that is needed for cell cycle progression. Expression of noncleavable talin blocks cell growth, adhesion maturation, proper mechanosensing, and the related property of EGF activation of motility. Further, the expression of talin rod in the presence of noncleavable full-length talin rescues cell growth and other functions. The cleavage of talin is found in early adhesions where there is also rapid turnover of talin that depends upon calpain and TRPM4 activity as well as the generation of force on talin. Thus, we suggest that an important function of talin is its control over cell cycle progression through its cleavage in early adhesions.

Original languageEnglish (US)
Pages (from-to)7242-7251
Number of pages10
JournalNano Letters
Issue number12
StatePublished - Dec 13 2017
Externally publishedYes


  • Adhesions
  • cell growth
  • mechanosensing
  • rigidity sensing
  • talin cleavage

ASJC Scopus subject areas

  • Bioengineering
  • General Chemistry
  • General Materials Science
  • Condensed Matter Physics
  • Mechanical Engineering


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