Force transduction by Triton cytoskeletons

Yasuhiro Sawada, Michael Sheetz

Research output: Contribution to journalArticle

239 Citations (Scopus)

Abstract

Force-initiated signal transduction can occur either via membrane-based ionic mechanisms or through changes in cytoskeletal-matrix linkages. We report here the stretch-dependent binding of cytoplasmic proteins to Triton X-100 cytoskeletons of L-929 cells grown on collagen-coated silicone. Triton X-100-insoluble cytoskeletons were stretched by 10% and incubated with biotinylated cytoplasmic proteins. Analysis with two-dimensional gel electrophoresis showed stretch-dependent binding of more than 10 cytoplasmic protein spots. Bound cytoplasmic proteins were purified by a photocleavable biotin tag and stretch-dependent binding of paxillin, focal adhesion kinase, and p130Cas was found, whereas the binding of vinculin was unchanged and actin binding decreased with stretch. Paxillin binding upon stretch was morphologically and biochemically similar in vitro and in vivo, that is, enhanced in the periphery and inhibited by the tyrosine phosphatase inhibitor, phenylarsine oxide. Thus, we suggest that transduction of matrix forces occurs through force-dependent conformation changes in the integrated cytoskeleton.

Original languageEnglish (US)
Pages (from-to)609-615
Number of pages7
JournalJournal of Cell Biology
Volume156
Issue number4
DOIs
StatePublished - Feb 18 2002
Externally publishedYes

Fingerprint

Cytoskeleton
Paxillin
Octoxynol
Vinculin
Focal Adhesion Protein-Tyrosine Kinases
Proteins
Electrophoresis, Gel, Two-Dimensional
Silicones
Biotin
Phosphoric Monoester Hydrolases
Tyrosine
Actins
Signal Transduction
Carrier Proteins
Collagen
Membranes

Keywords

  • Cytoskeleton
  • Focal contact
  • Force
  • Mechanotransduction
  • Tyrosine phosphatase

ASJC Scopus subject areas

  • Cell Biology

Cite this

Force transduction by Triton cytoskeletons. / Sawada, Yasuhiro; Sheetz, Michael.

In: Journal of Cell Biology, Vol. 156, No. 4, 18.02.2002, p. 609-615.

Research output: Contribution to journalArticle

Sawada, Yasuhiro ; Sheetz, Michael. / Force transduction by Triton cytoskeletons. In: Journal of Cell Biology. 2002 ; Vol. 156, No. 4. pp. 609-615.
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