Formaldehyde-protein conjugate-specific antibodies in rats exposed to formaldehyde

Hui Li, Jianling Wang, Rolf König, Ghulam Ansari, M Khan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A large human population is exposed to formaldehyde (FA) environmentally and occupationally, leading to a variety of respiratory and dermatological disturbances. FA covalently binds with proteins to form FA-protein conjugates, which might lead to the formation of FA-specific antibodies. The focus of this investigation was to study the formation of antibodies against FA-protein conjugates in rats for their possible use as biological markers of FA exposure. Male Sprague-Dawley rats were fed FA via drinking water (1.6 mg/ml) for up to 6 mo. Blood was collected at 3 and 6 mo following FA exposure, and formation of anti-FA-albumin adduct (anti-FAA) antibodies measured in the serum samples (1:100 dilution) by an enzyme-linked immunosorbent assay (ELISA) using synthesized rat albumin conjugates of FA as the solid-phase antigen. Sera from FA-treated rats showed induction of antibodies to FAA in 50% of the animals at both 3 and 6 mo, and the antibody titer was higher at 6 mo, suggesting a greater antibody response with exposure period. These antibodies were highly specific for FAA as they did not cross-react with malondialdehyde-, 4-hydroxynonenal-, 4-hydroxyhexenal-, and acrolein-albumin adducts. The specificity of anti-FAA antibodies was further evaluated by inhibition studies that showed a dose-dependent decrease in binding when the serum was preincubated with increasing concentrations of FAA, and by Western blot analysis that showed immunoreactivity of the antibody with FAA but not with rat albumin. Furthermore, the anti-FAA antibodies (rat serum) also recognized FA-human albumin (FAHA) conjugates, but had only approximately one-third of the binding affinity in comparison to FAA. Induction of anti-FA-protein conjugate antibodies could be further evaluated to serve as a biomarker of FA exposure.

Original languageEnglish (US)
Pages (from-to)1071-1075
Number of pages5
JournalJournal of Toxicology and Environmental Health - Part A: Current Issues
Volume70
Issue number13
DOIs
StatePublished - Jan 2007

Fingerprint

Formaldehyde
Rats
Antibodies
Albumins
Proteins
Serum
Antibody Formation
Biomarkers
Acrolein
Malondialdehyde
Immunosorbents
Drinking Water
Sprague Dawley Rats
Western Blotting
Enzyme-Linked Immunosorbent Assay
Dilution
Assays
Antigens
Animals
Blood

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Formaldehyde-protein conjugate-specific antibodies in rats exposed to formaldehyde. / Li, Hui; Wang, Jianling; König, Rolf; Ansari, Ghulam; Khan, M.

In: Journal of Toxicology and Environmental Health - Part A: Current Issues, Vol. 70, No. 13, 01.2007, p. 1071-1075.

Research output: Contribution to journalArticle

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abstract = "A large human population is exposed to formaldehyde (FA) environmentally and occupationally, leading to a variety of respiratory and dermatological disturbances. FA covalently binds with proteins to form FA-protein conjugates, which might lead to the formation of FA-specific antibodies. The focus of this investigation was to study the formation of antibodies against FA-protein conjugates in rats for their possible use as biological markers of FA exposure. Male Sprague-Dawley rats were fed FA via drinking water (1.6 mg/ml) for up to 6 mo. Blood was collected at 3 and 6 mo following FA exposure, and formation of anti-FA-albumin adduct (anti-FAA) antibodies measured in the serum samples (1:100 dilution) by an enzyme-linked immunosorbent assay (ELISA) using synthesized rat albumin conjugates of FA as the solid-phase antigen. Sera from FA-treated rats showed induction of antibodies to FAA in 50{\%} of the animals at both 3 and 6 mo, and the antibody titer was higher at 6 mo, suggesting a greater antibody response with exposure period. These antibodies were highly specific for FAA as they did not cross-react with malondialdehyde-, 4-hydroxynonenal-, 4-hydroxyhexenal-, and acrolein-albumin adducts. The specificity of anti-FAA antibodies was further evaluated by inhibition studies that showed a dose-dependent decrease in binding when the serum was preincubated with increasing concentrations of FAA, and by Western blot analysis that showed immunoreactivity of the antibody with FAA but not with rat albumin. Furthermore, the anti-FAA antibodies (rat serum) also recognized FA-human albumin (FAHA) conjugates, but had only approximately one-third of the binding affinity in comparison to FAA. Induction of anti-FA-protein conjugate antibodies could be further evaluated to serve as a biomarker of FA exposure.",
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