Formation and composition of the peritrophic membrane in the sand fly, Phlebotomus perniciosus (Diptera

Psychodidae).

L. L. Walters, K. P. Irons, H. Guzman, R. B. Tesh

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The secretion, morphology, and chemical composition of the peritrophic membrane was studied in the sand fly, Phlebotomus perniciosus Newstead. The membrane was secreted from the entire midgut epithelium. An electron-dense fine granular secretion, possibly chitin, was present along the length of the microvilli immediately until 24 h after feeding. From 12-48 h, an electron-lucid coarse granular component, possibly protein, was also secreted from the microvillar surface. By light microscopy, the mature 36-h membrane characteristically consisted of a dark anterior cap and posterior open ring, with a transparent intervening membrane and anterior plug. Ultrastructure of the fully formed membrane at 24-48 h was highly variable. Undifferentiated membranes appeared as a single electron-lucid layer; differentiated membranes were more complex, sometimes two-layered, containing electron-lucid and -dense fibers and granules. Results of binding to succinylated wheat germ agglutinin, histochemistry, and amino acid analysis indicated that the membrane was composed of chitin, glycoprotein, and protein. Eighteen amino acids were identified in membrane proteins; aspartic-glutamic acids (and amides), serine, glycine, and lysine (45% by weight) may be important in cross-linking membrane components.

Original languageEnglish (US)
Pages (from-to)179-198
Number of pages20
JournalJournal of Medical Entomology
Volume30
Issue number1
StatePublished - Jan 1993
Externally publishedYes

Fingerprint

Phlebotomus perniciosus
Phlebotomus
peritrophic membrane
Psychodidae
Phlebotominae
Diptera
electrons
Membranes
chitin
secretion
Electrons
amino acids
wheat germ
agglutinins
histochemistry
Chitin
amides
microvilli
glutamic acid
crosslinking

ASJC Scopus subject areas

  • Insect Science
  • veterinary(all)

Cite this

Formation and composition of the peritrophic membrane in the sand fly, Phlebotomus perniciosus (Diptera : Psychodidae). / Walters, L. L.; Irons, K. P.; Guzman, H.; Tesh, R. B.

In: Journal of Medical Entomology, Vol. 30, No. 1, 01.1993, p. 179-198.

Research output: Contribution to journalArticle

Walters, L. L. ; Irons, K. P. ; Guzman, H. ; Tesh, R. B. / Formation and composition of the peritrophic membrane in the sand fly, Phlebotomus perniciosus (Diptera : Psychodidae). In: Journal of Medical Entomology. 1993 ; Vol. 30, No. 1. pp. 179-198.
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