Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase, sorbitol dehydrogenase and sorbitol kinase

Satish Srivastava, Naseem Ansari, Brown James H., Mark Petrash J.

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase and sorbitol dehydrogenase by the reduction of glucose 6-phosphate and fructose 6-phosphate, respectively, has been demonstrated. The reaction product has been identified by Dowex-formate column chromatography, gas chromatography and mass spectrometry. Sorbitol 6-phosphate can also be formed by the phosphorylation of sorbitol by lens sorbitol kinase in the presence of ATP.

Original languageEnglish (US)
Pages (from-to)210-214
Number of pages5
JournalBBA - General Subjects
Volume717
Issue number2
DOIs
StatePublished - Aug 6 1982

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L-Iditol 2-Dehydrogenase
Aldehyde Reductase
formic acid
Lenses
Glucose-6-Phosphate
Column chromatography
Phosphorylation
Sorbitol
Reaction products
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Chromatography
Adenosine Triphosphate
sorbitol 6-phosphate
sorbitol kinase
Dowex
fructose-6-phosphate

Keywords

  • (Lens)
  • Aldose reductase
  • Sorbitol 6-phosphate
  • Sorbitol dehydrogenase
  • Sorbitol kinase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Medicine(all)

Cite this

Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase, sorbitol dehydrogenase and sorbitol kinase. / Srivastava, Satish; Ansari, Naseem; James H., Brown; J., Mark Petrash.

In: BBA - General Subjects, Vol. 717, No. 2, 06.08.1982, p. 210-214.

Research output: Contribution to journalArticle

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