Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase, sorbitol dehydrogenase and sorbitol kinase

Satish K. Srivastava, Ansari Naseem H., Brown James H., Mark Petrash J.

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase and sorbitol dehydrogenase by the reduction of glucose 6-phosphate and fructose 6-phosphate, respectively, has been demonstrated. The reaction product has been identified by Dowex-formate column chromatography, gas chromatography and mass spectrometry. Sorbitol 6-phosphate can also be formed by the phosphorylation of sorbitol by lens sorbitol kinase in the presence of ATP.

Original languageEnglish (US)
Pages (from-to)210-214
Number of pages5
JournalBBA - General Subjects
Volume717
Issue number2
DOIs
StatePublished - Aug 6 1982

Keywords

  • (Lens)
  • Aldose reductase
  • Sorbitol 6-phosphate
  • Sorbitol dehydrogenase
  • Sorbitol kinase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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