Formin DAAM1 organizes actin filaments in the cytoplasmic nodal actin network

Weiwei Luo, Zi Zhao Lieu, Ed Manser, Alexander D. Bershadsky, Michael Sheetz

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A nodal cytoplasmic actin network underlies actin cytoplasm cohesion in the absence of stress fibers. We previously described such a network that forms upon Latrunculin A (LatA) treatment, in which formin DAAM1 was localized at these nodes. Knock down of DAAM1 reduced the mobility of actin nodes but the nodes remained. Here we have investigated DAAM1 containing nodes after LatA washout. DAAM1 was found to be distributed between the cytoplasm and the plasma membrane. The membrane binding likely occurs through an interaction with lipid rafts, but is not required for F-actin assembly. Interesting the forced interaction of DAAM1 with plasma membrane through a rapamycin-dependent linkage, enhanced F-actin assembly at the cell membrane (compared to the cytoplasm) after the LatA washout. However, immediately after addition of both rapamycin and LatA, the cytoplasmic actin nodes formed transiently, before DAAM1 moved to the membrane. This was consistent with the idea that DAAM1 was initially anchored to cytoplasmic actin nodes. Further, photoactivatable tracking of DAAM1 showed DAAM1 was immobilized at these actin nodes. Thus, we suggest that DAAM1 organizes actin filaments into a nodal complex, and such nodal complexes seed actin network recovery after actin depolymerization.

Original languageEnglish (US)
Article numbere0163915
JournalPloS one
Volume11
Issue number10
DOIs
StatePublished - Oct 1 2016
Externally publishedYes

Fingerprint

microfilaments
Actin Cytoskeleton
actin
Actins
Cell membranes
Cytoplasm
cytoplasm
Cell Membrane
Sirolimus
plasma membrane
Stress Fibers
Membranes
depolymerization
Depolymerization
cohesion
cell membranes
Seeds
Seed
Lipids
Recovery

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Formin DAAM1 organizes actin filaments in the cytoplasmic nodal actin network. / Luo, Weiwei; Lieu, Zi Zhao; Manser, Ed; Bershadsky, Alexander D.; Sheetz, Michael.

In: PloS one, Vol. 11, No. 10, e0163915, 01.10.2016.

Research output: Contribution to journalArticle

Luo, Weiwei ; Lieu, Zi Zhao ; Manser, Ed ; Bershadsky, Alexander D. ; Sheetz, Michael. / Formin DAAM1 organizes actin filaments in the cytoplasmic nodal actin network. In: PloS one. 2016 ; Vol. 11, No. 10.
@article{99d58166738945a49494b31f8489e36a,
title = "Formin DAAM1 organizes actin filaments in the cytoplasmic nodal actin network",
abstract = "A nodal cytoplasmic actin network underlies actin cytoplasm cohesion in the absence of stress fibers. We previously described such a network that forms upon Latrunculin A (LatA) treatment, in which formin DAAM1 was localized at these nodes. Knock down of DAAM1 reduced the mobility of actin nodes but the nodes remained. Here we have investigated DAAM1 containing nodes after LatA washout. DAAM1 was found to be distributed between the cytoplasm and the plasma membrane. The membrane binding likely occurs through an interaction with lipid rafts, but is not required for F-actin assembly. Interesting the forced interaction of DAAM1 with plasma membrane through a rapamycin-dependent linkage, enhanced F-actin assembly at the cell membrane (compared to the cytoplasm) after the LatA washout. However, immediately after addition of both rapamycin and LatA, the cytoplasmic actin nodes formed transiently, before DAAM1 moved to the membrane. This was consistent with the idea that DAAM1 was initially anchored to cytoplasmic actin nodes. Further, photoactivatable tracking of DAAM1 showed DAAM1 was immobilized at these actin nodes. Thus, we suggest that DAAM1 organizes actin filaments into a nodal complex, and such nodal complexes seed actin network recovery after actin depolymerization.",
author = "Weiwei Luo and Lieu, {Zi Zhao} and Ed Manser and Bershadsky, {Alexander D.} and Michael Sheetz",
year = "2016",
month = "10",
day = "1",
doi = "10.1371/journal.pone.0163915",
language = "English (US)",
volume = "11",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "10",

}

TY - JOUR

T1 - Formin DAAM1 organizes actin filaments in the cytoplasmic nodal actin network

AU - Luo, Weiwei

AU - Lieu, Zi Zhao

AU - Manser, Ed

AU - Bershadsky, Alexander D.

AU - Sheetz, Michael

PY - 2016/10/1

Y1 - 2016/10/1

N2 - A nodal cytoplasmic actin network underlies actin cytoplasm cohesion in the absence of stress fibers. We previously described such a network that forms upon Latrunculin A (LatA) treatment, in which formin DAAM1 was localized at these nodes. Knock down of DAAM1 reduced the mobility of actin nodes but the nodes remained. Here we have investigated DAAM1 containing nodes after LatA washout. DAAM1 was found to be distributed between the cytoplasm and the plasma membrane. The membrane binding likely occurs through an interaction with lipid rafts, but is not required for F-actin assembly. Interesting the forced interaction of DAAM1 with plasma membrane through a rapamycin-dependent linkage, enhanced F-actin assembly at the cell membrane (compared to the cytoplasm) after the LatA washout. However, immediately after addition of both rapamycin and LatA, the cytoplasmic actin nodes formed transiently, before DAAM1 moved to the membrane. This was consistent with the idea that DAAM1 was initially anchored to cytoplasmic actin nodes. Further, photoactivatable tracking of DAAM1 showed DAAM1 was immobilized at these actin nodes. Thus, we suggest that DAAM1 organizes actin filaments into a nodal complex, and such nodal complexes seed actin network recovery after actin depolymerization.

AB - A nodal cytoplasmic actin network underlies actin cytoplasm cohesion in the absence of stress fibers. We previously described such a network that forms upon Latrunculin A (LatA) treatment, in which formin DAAM1 was localized at these nodes. Knock down of DAAM1 reduced the mobility of actin nodes but the nodes remained. Here we have investigated DAAM1 containing nodes after LatA washout. DAAM1 was found to be distributed between the cytoplasm and the plasma membrane. The membrane binding likely occurs through an interaction with lipid rafts, but is not required for F-actin assembly. Interesting the forced interaction of DAAM1 with plasma membrane through a rapamycin-dependent linkage, enhanced F-actin assembly at the cell membrane (compared to the cytoplasm) after the LatA washout. However, immediately after addition of both rapamycin and LatA, the cytoplasmic actin nodes formed transiently, before DAAM1 moved to the membrane. This was consistent with the idea that DAAM1 was initially anchored to cytoplasmic actin nodes. Further, photoactivatable tracking of DAAM1 showed DAAM1 was immobilized at these actin nodes. Thus, we suggest that DAAM1 organizes actin filaments into a nodal complex, and such nodal complexes seed actin network recovery after actin depolymerization.

UR - http://www.scopus.com/inward/record.url?scp=84992036738&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84992036738&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0163915

DO - 10.1371/journal.pone.0163915

M3 - Article

C2 - 27760153

AN - SCOPUS:84992036738

VL - 11

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 10

M1 - e0163915

ER -