Forward transport of glycoproteins on leading lamellipodia in locomoting cells

Dennis F. Kucik, Elliot L. Elson, Michael Sheetz

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

IN several types of locomoting cells, active rearward transport of particles on the cell surface has been observed and correlated with motility1-4. No forward transport of particles has previously been reported, however. Here we report rapid forward transport of concanavalin A-coated gold particles on the dorsal surfaces of lamellipodia of fish epidermal keratocytes. These movements are active, not diffusive, and more rapid than either rearward particle transport or the rate of cell locomotion. We observed forward transport in migrating, but not in stationary cells, and could block the movement by treatment with cytochalasin D. These studies demonstrate for the first time that small numbers of glycoproteins can be actively transported on the surface of the cell to the front of the lamellipodium. We suggest that this mechanism transports proteins involved in cell locomotion, such as proteins necessary for adhesion, and could also produce an extensile force.

Original languageEnglish (US)
Pages (from-to)315-317
Number of pages3
JournalNature
Volume340
Issue number6231
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

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Pseudopodia
Glycoproteins
Cell Movement
Cytochalasin D
Active Biological Transport
Carrier Proteins
Fishes
Proteins

ASJC Scopus subject areas

  • General

Cite this

Forward transport of glycoproteins on leading lamellipodia in locomoting cells. / Kucik, Dennis F.; Elson, Elliot L.; Sheetz, Michael.

In: Nature, Vol. 340, No. 6231, 01.01.1989, p. 315-317.

Research output: Contribution to journalArticle

Kucik, Dennis F. ; Elson, Elliot L. ; Sheetz, Michael. / Forward transport of glycoproteins on leading lamellipodia in locomoting cells. In: Nature. 1989 ; Vol. 340, No. 6231. pp. 315-317.
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