Fractional synthetic rate of skeletal muscle protein during testosterone infusion

A. Ferrando, K. Tipton, D. Dovlc, S. Phillips, Joaquin Cortiella, R. Wolfe

Research output: Contribution to journalArticle

Abstract

Testosterone (T) administration is known to increase muscle anabolism. However, traditional injections may not be maximally effective in the burned patient due to increased lipogenesis and lipid storage. Therefore, the effects of T infusion on skeletal muscle protein synthesis were investigated in normal male volunteers. Three subjects received 30 mg of methyl-testosterone infusion or the carrier (Intralipid) over a five hour period. Subjects were studied in a randomized manner and were blinded to the treatment. Muscle biopsies of the vastus lateralis were taken at 2 and 5 h of infusion. Fractional synthetic rate (FSR) was calculated based on the precursor-product method. The average intracellular enrichment over the time of incorporation was used as the precursor, whereas the change in protein-bound enrichment of ring-d, -phenylalanine from 2 to 5 h of infusion was the product. There was a trend toward increased FSR during T infusion (0.0801 0.018 (SD) vs 0.048 ±0.018 %/hr). However, this increase railed to reach statistical significance by paired t-test (p = 0.10). Contrary to our previous finding with chronic T injections, acute administration of T may not be a potent stimulator of muscle protein synthesis.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996

Fingerprint

Muscle Proteins
muscle protein
testosterone
Muscle
skeletal muscle
Testosterone
Skeletal Muscle
protein synthesis
injection
methyltestosterone
Muscles
muscles
Lipogenesis
Injections
Biopsy
Quadriceps Muscle
lipogenesis
Phenylalanine
phenylalanine
volunteers

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Ferrando, A., Tipton, K., Dovlc, D., Phillips, S., Cortiella, J., & Wolfe, R. (1996). Fractional synthetic rate of skeletal muscle protein during testosterone infusion. FASEB Journal, 10(3).

Fractional synthetic rate of skeletal muscle protein during testosterone infusion. / Ferrando, A.; Tipton, K.; Dovlc, D.; Phillips, S.; Cortiella, Joaquin; Wolfe, R.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

Ferrando, A, Tipton, K, Dovlc, D, Phillips, S, Cortiella, J & Wolfe, R 1996, 'Fractional synthetic rate of skeletal muscle protein during testosterone infusion', FASEB Journal, vol. 10, no. 3.
Ferrando, A. ; Tipton, K. ; Dovlc, D. ; Phillips, S. ; Cortiella, Joaquin ; Wolfe, R. / Fractional synthetic rate of skeletal muscle protein during testosterone infusion. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
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