Free radical scavenger depletion in post-ischemic reperfusion brain damage

A. Vanella, C. Di Giacomo, V. Sorrenti, A. Russo, C. Castorina, A. Campisi, M. Renis, J. R. Perez-Polo

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70 Scopus citations

Abstract

In the present study the influence of pretreatment with various GSH depletors such as buthionine sulfoximine (BSO) and diethylmaleate (DEM) was investigated in rats following cerebral postischemic reperfusion. Moreover, the effect of diethyldithiocarbamic acid (DDC), inhibitor of endogenous Cu,Zn-SOD, was evaluated. A significant depletion (40% of control value) of GSH levels was observed 24 h after DEM administration; after 48 h the value reached control levels. BSO showed maximal GSH depletion (59%) 24 h after administration and it was constant for almost 48 h. DDC administration caused a marked decrease (60%) of Cu,Zn-SOD activity 4 h after the injection and induced a marked decrease in percentage of survival with respect to control (untreated, ischemic) rats, when administered 4 h before ischemia. BSO and DEM prolonged the survival time of animals when administered 24 h before ischemia. This last paradoxical effect is unclear at present, but it might be due to an influence on glutamate cascade.

Original languageEnglish (US)
Pages (from-to)1337-1340
Number of pages4
JournalNeurochemical Research
Volume18
Issue number12
DOIs
StatePublished - Dec 1993
Externally publishedYes

Keywords

  • GSH-depletors
  • Ischemia
  • reperfusion
  • scavenger
  • survival

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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