Frequent gain of the p40/p51/p63 gene locus in primary head and neck squamous cell carcinoma

Kengo Yamaguchi, Li Wu, Otávia L. Caballero, Kenji Hibi, Barry Trink, Vicente Resto, Paul Cairns, Kenji Okami, Wayne M. Koch, David Sidransky, Jin Jen

Research output: Contribution to journalArticlepeer-review

114 Scopus citations

Abstract

We have identified a new human p53 homologue, p40 (p51/p63). This gene was mapped to the distal arm of 3q and was found to be essential for normal epithelial development. We used microsatellite and FISH analyses to search for genetic alterations of p40 in primary HNSCC. A more precise localization of p40 was completed using 6 known markers on 3q and a newly isolated microsatellite marker within the p40 gene. We also determined the genomic organization of the p40 gene using human YAC and BAC clones. Microsatellite analysis revealed that 14 of 26 (54%) primary HNSCC had allelic imbalance in at least 1 of the 7 microsatellite loci. However, FISH analysis with a p40 probe showed that a majority of HNSCC had an increased copy number of the locus regardless of allelic status. Thus, overrepresentation of the p40 locus may play an important role in the development of HNSCC. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)684-689
Number of pages6
JournalInternational Journal of Cancer
Volume86
Issue number5
DOIs
StatePublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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