Frozen human serum reference material for standardization of sodium and potassium measurements in serum or plasma by ion-selective electrode analyzers

Prema C. Gunaratna, William F. Koch, Robert C. Paule, Alan D. Cormier, Paul D'Orazio, Neil Greenberg, Kathleen M. O'Connell, Arthur Malenfant, Anthony Okorodudu, Richard Miller, Debra M. Kus, George N. Bowers

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Three interlaboratory round-robin studies (RR1, RR2, and RR3) were conducted to identify a serum-based reference material that would aid in the standardization of direct ion-selective electrode (ISE) measurements of sodium and potassium. Ultrafiltered frozen serum reference materials requiring no reconstitution reduced between-laboratory variability (the largest source of imprecision) more than did other reference materials. ISE values for RR3 were normalized by the use of two points at the extremes of the clinical range for sodium (i.e., 120 and 160 mmol/L), with values assigned by the flame atomic emission spectrometry (FAES) Reference Method. This FAES normalization of ISE raw values remarkably improved all sources of variability and unified the results from seven different direct ISE analyzers to the FAES Reference Method value. Subsequently, a three-tiered, fresh-frozen human serum reference material was produced to the specifications developed in RR1-RR3, was assigned certified values for sodium and potassium by Definitive Methods at the National Institute of Standards and Technology (NIST), and was made available in 1990 to the clinical laboratory community as a Standard Reference Material (SRM); it is now identified as SRM 956. Albeit retrospectively, we show how applying an FAES normalization step identical to that used in RR4/5 to the ISE data for SRM 956 after the NIST Definitive Method values were known, consistently moved the ISE results for RR3 closer to the true value for Na+ and K+.

Original languageEnglish (US)
Pages (from-to)1459-1465
Number of pages7
JournalClinical Chemistry
Volume38
Issue number8
StatePublished - 1992
Externally publishedYes

Fingerprint

Ion-Selective Electrodes
Standardization
Potassium
Sodium
Plasmas
Spectrum Analysis
Spectrometry
Serum
Technology
Clinical laboratories
Songbirds
Reference Values
Specifications

Keywords

  • Definitive method-assigned values
  • Electrolytes
  • Flame atomic emission spectrometry
  • National reference system
  • Reference material

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Gunaratna, P. C., Koch, W. F., Paule, R. C., Cormier, A. D., D'Orazio, P., Greenberg, N., ... Bowers, G. N. (1992). Frozen human serum reference material for standardization of sodium and potassium measurements in serum or plasma by ion-selective electrode analyzers. Clinical Chemistry, 38(8), 1459-1465.

Frozen human serum reference material for standardization of sodium and potassium measurements in serum or plasma by ion-selective electrode analyzers. / Gunaratna, Prema C.; Koch, William F.; Paule, Robert C.; Cormier, Alan D.; D'Orazio, Paul; Greenberg, Neil; O'Connell, Kathleen M.; Malenfant, Arthur; Okorodudu, Anthony; Miller, Richard; Kus, Debra M.; Bowers, George N.

In: Clinical Chemistry, Vol. 38, No. 8, 1992, p. 1459-1465.

Research output: Contribution to journalArticle

Gunaratna, PC, Koch, WF, Paule, RC, Cormier, AD, D'Orazio, P, Greenberg, N, O'Connell, KM, Malenfant, A, Okorodudu, A, Miller, R, Kus, DM & Bowers, GN 1992, 'Frozen human serum reference material for standardization of sodium and potassium measurements in serum or plasma by ion-selective electrode analyzers', Clinical Chemistry, vol. 38, no. 8, pp. 1459-1465.
Gunaratna, Prema C. ; Koch, William F. ; Paule, Robert C. ; Cormier, Alan D. ; D'Orazio, Paul ; Greenberg, Neil ; O'Connell, Kathleen M. ; Malenfant, Arthur ; Okorodudu, Anthony ; Miller, Richard ; Kus, Debra M. ; Bowers, George N. / Frozen human serum reference material for standardization of sodium and potassium measurements in serum or plasma by ion-selective electrode analyzers. In: Clinical Chemistry. 1992 ; Vol. 38, No. 8. pp. 1459-1465.
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AU - Gunaratna, Prema C.

AU - Koch, William F.

AU - Paule, Robert C.

AU - Cormier, Alan D.

AU - D'Orazio, Paul

AU - Greenberg, Neil

AU - O'Connell, Kathleen M.

AU - Malenfant, Arthur

AU - Okorodudu, Anthony

AU - Miller, Richard

AU - Kus, Debra M.

AU - Bowers, George N.

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