FT-ICR MS and MS/MS identification of sites of phosphorylation in Protein Kinase C α, δand ζ

Michael J. Chalmers, Harald Mischak, Simon J. Gaskell, Mark R. Emmett, Alan G. Marshall

Research output: Contribution to conferencePaper

Abstract

Identification of sites of phosphorylation in protein kinase was carried out using FT-ICR MS and MS/MS. A fast load capillary LC apparatus was occupied to a 9.4 T micro-EST FT-ICR mass spectrometer with external ion accumulation. The combination provides a powerful tool for the identification of peptides present at low stoichiometric ratios within complex mixtures. The use of electron capture dissociation for the location of phosphorylated residues was also evaluated.

Original languageEnglish (US)
Pages313-314
Number of pages2
StatePublished - Dec 1 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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    Chalmers, M. J., Mischak, H., Gaskell, S. J., Emmett, M. R., & Marshall, A. G. (2002). FT-ICR MS and MS/MS identification of sites of phosphorylation in Protein Kinase C α, δand ζ. 313-314. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.