FT-ICR MS and MS/MS identification of sites of phosphorylation in Protein Kinase C α, δand ζ

Michael J. Chalmers; Harald Mischak; Simon J. Gaskell; Mark R. Emmett; Alan G. Marshall

FT-ICR MS and MS/MS identification of sites of phosphorylation in Protein Kinase C α, δand ζ

Michael J. Chalmers, Harald Mischak, Simon J. Gaskell, Mark R. Emmett, Alan G. Marshall

Research output: Contribution to conference › Paper › peer-review

Abstract

Identification of sites of phosphorylation in protein kinase was carried out using FT-ICR MS and MS/MS. A fast load capillary LC apparatus was occupied to a 9.4 T micro-EST FT-ICR mass spectrometer with external ion accumulation. The combination provides a powerful tool for the identification of peptides present at low stoichiometric ratios within complex mixtures. The use of electron capture dissociation for the location of phosphorylated residues was also evaluated.

Original language	English (US)
Pages	313-314
Number of pages	2
State	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

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title = "FT-ICR MS and MS/MS identification of sites of phosphorylation in Protein Kinase C α, δand ζ",

abstract = "Identification of sites of phosphorylation in protein kinase was carried out using FT-ICR MS and MS/MS. A fast load capillary LC apparatus was occupied to a 9.4 T micro-EST FT-ICR mass spectrometer with external ion accumulation. The combination provides a powerful tool for the identification of peptides present at low stoichiometric ratios within complex mixtures. The use of electron capture dissociation for the location of phosphorylated residues was also evaluated.",

author = "Chalmers, {Michael J.} and Harald Mischak and Gaskell, {Simon J.} and Emmett, {Mark R.} and Marshall, {Alan G.}",

year = "2002",

language = "English (US)",

pages = "313--314",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - CONF

T1 - FT-ICR MS and MS/MS identification of sites of phosphorylation in Protein Kinase C α, δand ζ

AU - Chalmers, Michael J.

AU - Mischak, Harald

AU - Gaskell, Simon J.

AU - Emmett, Mark R.

AU - Marshall, Alan G.

PY - 2002

Y1 - 2002

N2 - Identification of sites of phosphorylation in protein kinase was carried out using FT-ICR MS and MS/MS. A fast load capillary LC apparatus was occupied to a 9.4 T micro-EST FT-ICR mass spectrometer with external ion accumulation. The combination provides a powerful tool for the identification of peptides present at low stoichiometric ratios within complex mixtures. The use of electron capture dissociation for the location of phosphorylated residues was also evaluated.

AB - Identification of sites of phosphorylation in protein kinase was carried out using FT-ICR MS and MS/MS. A fast load capillary LC apparatus was occupied to a 9.4 T micro-EST FT-ICR mass spectrometer with external ion accumulation. The combination provides a powerful tool for the identification of peptides present at low stoichiometric ratios within complex mixtures. The use of electron capture dissociation for the location of phosphorylated residues was also evaluated.

UR - http://www.scopus.com/inward/record.url?scp=2442663174&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442663174&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442663174

SP - 313

EP - 314

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

FT-ICR MS and MS/MS identification of sites of phosphorylation in Protein Kinase C α, δand ζ

Abstract

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ASJC Scopus subject areas

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