FT-ICR MS and MS/MS identification of sites of phosphorylation in Protein Kinase C α, δand ζ

Michael J. Chalmers, Harald Mischak, Simon J. Gaskell, Mark Emmett, Alan G. Marshall

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Identification of sites of phosphorylation in protein kinase was carried out using FT-ICR MS and MS/MS. A fast load capillary LC apparatus was occupied to a 9.4 T micro-EST FT-ICR mass spectrometer with external ion accumulation. The combination provides a powerful tool for the identification of peptides present at low stoichiometric ratios within complex mixtures. The use of electron capture dissociation for the location of phosphorylated residues was also evaluated.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages313-314
Number of pages2
StatePublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

Phosphorylation
Expressed Sequence Tags
Mass spectrometers
Complex Mixtures
Protein Kinases
Protein Kinase C
Ions
Peptides
Electrons

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Chalmers, M. J., Mischak, H., Gaskell, S. J., Emmett, M., & Marshall, A. G. (2002). FT-ICR MS and MS/MS identification of sites of phosphorylation in Protein Kinase C α, δand ζ. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 313-314)

FT-ICR MS and MS/MS identification of sites of phosphorylation in Protein Kinase C α, δand ζ. / Chalmers, Michael J.; Mischak, Harald; Gaskell, Simon J.; Emmett, Mark; Marshall, Alan G.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 313-314.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chalmers, MJ, Mischak, H, Gaskell, SJ, Emmett, M & Marshall, AG 2002, FT-ICR MS and MS/MS identification of sites of phosphorylation in Protein Kinase C α, δand ζ. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 313-314, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Chalmers MJ, Mischak H, Gaskell SJ, Emmett M, Marshall AG. FT-ICR MS and MS/MS identification of sites of phosphorylation in Protein Kinase C α, δand ζ. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 313-314
Chalmers, Michael J. ; Mischak, Harald ; Gaskell, Simon J. ; Emmett, Mark ; Marshall, Alan G. / FT-ICR MS and MS/MS identification of sites of phosphorylation in Protein Kinase C α, δand ζ. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 313-314
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