Abstract
Background: During the pandemic [Influenza A(H1N1)pdm09] period in 2009-2010, an influenza A (Inf-A) virus with H1N2 subtype (designated as A/Eastern India/N-1289/2009) was detected from a 25 years old male from Mizoram (North-eastern India). Objective. To characterize full genome of the H1N2 influenza virus. Methods. For initial detection of Influenza viruses, amplification of matrix protein (M) gene of Inf-A and B viruses was carried out by real time RT-PCR. Influenza A positive viruses are then further subtyped with HA and NA gene specific primers. Sequencing and the phylogenetic analysis was performed for the H1N2 strain to understand its origin. Results: The outcome of this full genome study revealed a unique reassortment event where the N-1289 virus acquired it's HA gene from a 2009 pandemic H1N1 virus with swine origin and the other genes from H3N2-like viruses of human origin. Conclusions: This study provides information on possibility of occurrence of reassortment events during influenza season when infectivity is high and two different subtypes of Inf-A viruses co-circulate in same geographical location.
| Original language | English (US) |
|---|---|
| Article number | 233 |
| Journal | Virology Journal |
| Volume | 9 |
| DOIs | |
| State | Published - 2012 |
| Externally published | Yes |
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Keywords
- Full genome
- H1N2
- India
- Influenza A
- Reassortment
ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
Full genomic analysis of an influenza A (H1N2) virus identified during 2009 pandemic in Eastern India : Evidence of reassortment event between co-circulating A(H1N1)pdm09 and A/Brisbane/10/2007-like H3N2 strains. / Mukherjee, Tapasi Roy; Agrawal, Anurodh S.; Chakrabarti, Sekhar; Chawla-Sarkar, Mamta.
In: Virology Journal, Vol. 9, 233, 2012.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Full genomic analysis of an influenza A (H1N2) virus identified during 2009 pandemic in Eastern India
T2 - Evidence of reassortment event between co-circulating A(H1N1)pdm09 and A/Brisbane/10/2007-like H3N2 strains
AU - Mukherjee, Tapasi Roy
AU - Agrawal, Anurodh S.
AU - Chakrabarti, Sekhar
AU - Chawla-Sarkar, Mamta
PY - 2012
Y1 - 2012
N2 - Background: During the pandemic [Influenza A(H1N1)pdm09] period in 2009-2010, an influenza A (Inf-A) virus with H1N2 subtype (designated as A/Eastern India/N-1289/2009) was detected from a 25 years old male from Mizoram (North-eastern India). Objective. To characterize full genome of the H1N2 influenza virus. Methods. For initial detection of Influenza viruses, amplification of matrix protein (M) gene of Inf-A and B viruses was carried out by real time RT-PCR. Influenza A positive viruses are then further subtyped with HA and NA gene specific primers. Sequencing and the phylogenetic analysis was performed for the H1N2 strain to understand its origin. Results: The outcome of this full genome study revealed a unique reassortment event where the N-1289 virus acquired it's HA gene from a 2009 pandemic H1N1 virus with swine origin and the other genes from H3N2-like viruses of human origin. Conclusions: This study provides information on possibility of occurrence of reassortment events during influenza season when infectivity is high and two different subtypes of Inf-A viruses co-circulate in same geographical location.
AB - Background: During the pandemic [Influenza A(H1N1)pdm09] period in 2009-2010, an influenza A (Inf-A) virus with H1N2 subtype (designated as A/Eastern India/N-1289/2009) was detected from a 25 years old male from Mizoram (North-eastern India). Objective. To characterize full genome of the H1N2 influenza virus. Methods. For initial detection of Influenza viruses, amplification of matrix protein (M) gene of Inf-A and B viruses was carried out by real time RT-PCR. Influenza A positive viruses are then further subtyped with HA and NA gene specific primers. Sequencing and the phylogenetic analysis was performed for the H1N2 strain to understand its origin. Results: The outcome of this full genome study revealed a unique reassortment event where the N-1289 virus acquired it's HA gene from a 2009 pandemic H1N1 virus with swine origin and the other genes from H3N2-like viruses of human origin. Conclusions: This study provides information on possibility of occurrence of reassortment events during influenza season when infectivity is high and two different subtypes of Inf-A viruses co-circulate in same geographical location.
KW - Full genome
KW - H1N2
KW - India
KW - Influenza A
KW - Reassortment
UR - http://www.scopus.com/inward/record.url?scp=84874108210&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84874108210&partnerID=8YFLogxK
U2 - 10.1186/1743-422X-9-233
DO - 10.1186/1743-422X-9-233
M3 - Article
C2 - 23057530
AN - SCOPUS:84874108210
VL - 9
JO - Virology Journal
JF - Virology Journal
SN - 1743-422X
M1 - 233
ER -