Full genomic analysis of an influenza A (H1N2) virus identified during 2009 pandemic in Eastern India

Evidence of reassortment event between co-circulating A(H1N1)pdm09 and A/Brisbane/10/2007-like H3N2 strains

Tapasi Roy Mukherjee, Anurodh S. Agrawal, Sekhar Chakrabarti, Mamta Chawla-Sarkar

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: During the pandemic [Influenza A(H1N1)pdm09] period in 2009-2010, an influenza A (Inf-A) virus with H1N2 subtype (designated as A/Eastern India/N-1289/2009) was detected from a 25 years old male from Mizoram (North-eastern India). Objective. To characterize full genome of the H1N2 influenza virus. Methods. For initial detection of Influenza viruses, amplification of matrix protein (M) gene of Inf-A and B viruses was carried out by real time RT-PCR. Influenza A positive viruses are then further subtyped with HA and NA gene specific primers. Sequencing and the phylogenetic analysis was performed for the H1N2 strain to understand its origin. Results: The outcome of this full genome study revealed a unique reassortment event where the N-1289 virus acquired it's HA gene from a 2009 pandemic H1N1 virus with swine origin and the other genes from H3N2-like viruses of human origin. Conclusions: This study provides information on possibility of occurrence of reassortment events during influenza season when infectivity is high and two different subtypes of Inf-A viruses co-circulate in same geographical location.

Original languageEnglish (US)
Article number233
JournalVirology Journal
Volume9
DOIs
StatePublished - 2012
Externally publishedYes

Fingerprint

H1N2 Subtype Influenza A Virus
Influenza A virus
Pandemics
India
Orthomyxoviridae
Human Influenza
Genome
H3N2 Subtype Influenza A Virus
Genes
Influenza B virus
H1N1 Subtype Influenza A Virus
Real-Time Polymerase Chain Reaction
Swine
Viruses
Proteins

Keywords

  • Full genome
  • H1N2
  • India
  • Influenza A
  • Reassortment

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Full genomic analysis of an influenza A (H1N2) virus identified during 2009 pandemic in Eastern India : Evidence of reassortment event between co-circulating A(H1N1)pdm09 and A/Brisbane/10/2007-like H3N2 strains. / Mukherjee, Tapasi Roy; Agrawal, Anurodh S.; Chakrabarti, Sekhar; Chawla-Sarkar, Mamta.

In: Virology Journal, Vol. 9, 233, 2012.

Research output: Contribution to journalArticle

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