Function of bacteriophage G7C esterase tailspike in host cell adsorption

Nikolai Prokhorov, Cristian Riccio, Evelina L. Zdorovenko, Mikhail M. Shneider, Christopher Browning, Yuriy A. Knirel, Petr Leiman, Andrey V. Letarov

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Bacteriophages recognize and bind to their hosts with the help of receptor-binding proteins (RBPs) that emanate from the phage particle in the form of fibers or tailspikes. RBPs show a great variability in their shapes, sizes, and location on the particle. Some RBPs are known to depolymerize surface polysaccharides of the host while others show no enzymatic activity. Here we report that both RBPs of podovirus G7C - tailspikes gp63.1 and gp66 - are essential for infection of its natural host bacterium E. coli 4s that populates the equine intestinal tract. We characterize the structure and function of gp63.1 and show that unlike any previously described RPB, gp63.1 deacetylates surface polysaccharides of E. coli 4s leaving the backbone of the polysaccharide intact. We demonstrate that gp63.1 and gp66 form a stable complex, in which the N-terminal part of gp66 serves as an attachment site for gp63.1 and anchors the gp63.1-gp66 complex to the G7C tail. The esterase domain of gp63.1 as well as domains mediating the gp63.1-gp66 interaction is widespread among all three families of tailed bacteriophages.

Original languageEnglish (US)
JournalMolecular Microbiology
DOIs
StateAccepted/In press - 2017

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Esterases
Bacteriophages
Adsorption
Carrier Proteins
Polysaccharides
Escherichia coli
Horses
Bacteria
Infection

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Prokhorov, N., Riccio, C., Zdorovenko, E. L., Shneider, M. M., Browning, C., Knirel, Y. A., ... Letarov, A. V. (Accepted/In press). Function of bacteriophage G7C esterase tailspike in host cell adsorption. Molecular Microbiology. https://doi.org/10.1111/mmi.13710

Function of bacteriophage G7C esterase tailspike in host cell adsorption. / Prokhorov, Nikolai; Riccio, Cristian; Zdorovenko, Evelina L.; Shneider, Mikhail M.; Browning, Christopher; Knirel, Yuriy A.; Leiman, Petr; Letarov, Andrey V.

In: Molecular Microbiology, 2017.

Research output: Contribution to journalArticle

Prokhorov, N, Riccio, C, Zdorovenko, EL, Shneider, MM, Browning, C, Knirel, YA, Leiman, P & Letarov, AV 2017, 'Function of bacteriophage G7C esterase tailspike in host cell adsorption', Molecular Microbiology. https://doi.org/10.1111/mmi.13710
Prokhorov N, Riccio C, Zdorovenko EL, Shneider MM, Browning C, Knirel YA et al. Function of bacteriophage G7C esterase tailspike in host cell adsorption. Molecular Microbiology. 2017. https://doi.org/10.1111/mmi.13710
Prokhorov, Nikolai ; Riccio, Cristian ; Zdorovenko, Evelina L. ; Shneider, Mikhail M. ; Browning, Christopher ; Knirel, Yuriy A. ; Leiman, Petr ; Letarov, Andrey V. / Function of bacteriophage G7C esterase tailspike in host cell adsorption. In: Molecular Microbiology. 2017.
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