Functional Analysis of Glycosylation of Zika Virus Envelope Protein

Camila R. Fontes-Garfias, Chao Shan, Huanle Luo, Antonio E. Muruato, Daniele B.A. Medeiros, Elizabeth Mays, Xuping Xie, Jing Zou, Christopher M. Roundy, Maki Wakamiya, Shannan Rossi, Tian Wang, Scott Weaver, Pei-Yong Shi

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Zika virus (ZIKV) infection causes devastating congenital abnormities and Guillain-Barré syndrome. The ZIKV envelope (E) protein is responsible for viral entry and represents a major determinant for viral pathogenesis. Like other flaviviruses, the ZIKV E protein is glycosylated at amino acid N154. To study the function of E glycosylation, we generated a recombinant N154Q ZIKV that lacks the E glycosylation and analyzed the mutant virus in mammalian and mosquito hosts. In mouse models, the mutant was attenuated, as evidenced by lower viremia, decreased weight loss, and no mortality; however, knockout of E glycosylation did not significantly affect neurovirulence. Mice immunized with the mutant virus developed a robust neutralizing antibody response and were completely protected from wild-type ZIKV challenge. In mosquitoes, the mutant virus exhibited diminished oral infectivity for the Aedes aegypti vector. Collectively, the results demonstrate that E glycosylation is critical for ZIKV infection of mammalian and mosquito hosts. Zika virus (ZIKV) causes devastating congenital abnormities and Guillain-Barré syndrome. Fontes-Garfias et al. showed that the glycosylation of ZIKV envelope protein plays an important role in infecting mosquito vectors and pathogenesis in mouse.

Original languageEnglish (US)
Pages (from-to)1180-1190
Number of pages11
JournalCell Reports
Volume21
Issue number5
DOIs
StatePublished - Oct 31 2017

Fingerprint

Viral Envelope Proteins
Glycosylation
Functional analysis
Viruses
Culicidae
Flavivirus
Aedes
Viremia
Neutralizing Antibodies
Antibody Formation
Zika Virus
Weight Loss
Amino Acids
Mortality
Proteins

Keywords

  • flavivirus entry
  • glycosylation
  • mosquito transmission
  • vaccine
  • Zika virus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Fontes-Garfias, C. R., Shan, C., Luo, H., Muruato, A. E., Medeiros, D. B. A., Mays, E., ... Shi, P-Y. (2017). Functional Analysis of Glycosylation of Zika Virus Envelope Protein. Cell Reports, 21(5), 1180-1190. https://doi.org/10.1016/j.celrep.2017.10.016

Functional Analysis of Glycosylation of Zika Virus Envelope Protein. / Fontes-Garfias, Camila R.; Shan, Chao; Luo, Huanle; Muruato, Antonio E.; Medeiros, Daniele B.A.; Mays, Elizabeth; Xie, Xuping; Zou, Jing; Roundy, Christopher M.; Wakamiya, Maki; Rossi, Shannan; Wang, Tian; Weaver, Scott; Shi, Pei-Yong.

In: Cell Reports, Vol. 21, No. 5, 31.10.2017, p. 1180-1190.

Research output: Contribution to journalArticle

Fontes-Garfias, CR, Shan, C, Luo, H, Muruato, AE, Medeiros, DBA, Mays, E, Xie, X, Zou, J, Roundy, CM, Wakamiya, M, Rossi, S, Wang, T, Weaver, S & Shi, P-Y 2017, 'Functional Analysis of Glycosylation of Zika Virus Envelope Protein', Cell Reports, vol. 21, no. 5, pp. 1180-1190. https://doi.org/10.1016/j.celrep.2017.10.016
Fontes-Garfias CR, Shan C, Luo H, Muruato AE, Medeiros DBA, Mays E et al. Functional Analysis of Glycosylation of Zika Virus Envelope Protein. Cell Reports. 2017 Oct 31;21(5):1180-1190. https://doi.org/10.1016/j.celrep.2017.10.016
Fontes-Garfias, Camila R. ; Shan, Chao ; Luo, Huanle ; Muruato, Antonio E. ; Medeiros, Daniele B.A. ; Mays, Elizabeth ; Xie, Xuping ; Zou, Jing ; Roundy, Christopher M. ; Wakamiya, Maki ; Rossi, Shannan ; Wang, Tian ; Weaver, Scott ; Shi, Pei-Yong. / Functional Analysis of Glycosylation of Zika Virus Envelope Protein. In: Cell Reports. 2017 ; Vol. 21, No. 5. pp. 1180-1190.
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