Functional and ligand binding specificity of the rabbit neutrophil IL-8 receptor

Kathleen M. Thomas, Linda Taylor, Gregory Prado, Jose Romero, Bernhard Moser, Bruce Car, Alfred Walz, Marco Baggiolini, Javier Navarro

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Abstract

IL-8 mediates migration and activation of neutrophils. This study describes the functional and ligand binding specificity of the human intercrine peptides IL-8, neutrophil-activating peptide 2 (NAP-2), melanoma growth stimulatory activity (GRO), and platelet factor 4 (PF4) to rabbit neutrophils and mammalian cell lines transfected with rabbit IL-8 receptor cDNA (F3R). Rabbit neutrophil membranes bound 125I-labeled IL-8 and 125I-labeled NAP-2 but did not bind 125I-labeled GRO or 125I- labeled PF4. Rabbit neutrophils mobilized intracellular Ca2+ in response to IL-8 and NAP-2 but not to GRO or PF4. Monkey kidney cells (COS-7) and hamster lung fibroblasts (CCL-39) were transiently and stably transfected with the rabbit neutrophil IL-8 receptor F3R cDNA. COS-7 cells transfected with F3R cDNA bound 125I-labeled IL-8 but did not bind other IL-8-related peptides such as 125I-labeled NAP-2, 125I-labeled GRO, or 125I-labeled PF4. Furthermore, bound 125I-labeled IL-8 was only displaced by unlabeled IL-8 but not by unlabeled NAP-2, GROα, or PF4. Consistent with this observation, stably transfected CCL 39 cells expressing F3R cDNA mobilized Ca2+ only in response to IL-8. We conclude that F3R cDNA encodes a functional IL-8 receptor isotype with strict ligand binding specificity for IL-8, that rabbit neutrophils do not bind human GROα, and it is suggested that rabbit neutrophils contain in addition to the F3R protein another IL-8 receptor isotype with broad ligand specificity or a distinct NAP-2 receptor.

Original languageEnglish (US)
Pages (from-to)2496-2500
Number of pages5
JournalJournal of Immunology
Volume152
Issue number5
StatePublished - Mar 1 1994

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Thomas, K. M., Taylor, L., Prado, G., Romero, J., Moser, B., Car, B., Walz, A., Baggiolini, M., & Navarro, J. (1994). Functional and ligand binding specificity of the rabbit neutrophil IL-8 receptor. Journal of Immunology, 152(5), 2496-2500.