Functional and ligand binding specificity of the rabbit neutrophil IL-8 receptor

Kathleen M. Thomas, Linda Taylor, Gregory Prado, Jose Romero, Bernhard Moser, Bruce Car, Alfred Walz, Marco Baggiolini, Javier Navarro

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

IL-8 mediates migration and activation of neutrophils. This study describes the functional and ligand binding specificity of the human intercrine peptides IL-8, neutrophil-activating peptide 2 (NAP-2), melanoma growth stimulatory activity (GRO), and platelet factor 4 (PF4) to rabbit neutrophils and mammalian cell lines transfected with rabbit IL-8 receptor cDNA (F3R). Rabbit neutrophil membranes bound 125I-labeled IL-8 and 125I-labeled NAP-2 but did not bind 125I-labeled GRO or 125I- labeled PF4. Rabbit neutrophils mobilized intracellular Ca2+ in response to IL-8 and NAP-2 but not to GRO or PF4. Monkey kidney cells (COS-7) and hamster lung fibroblasts (CCL-39) were transiently and stably transfected with the rabbit neutrophil IL-8 receptor F3R cDNA. COS-7 cells transfected with F3R cDNA bound 125I-labeled IL-8 but did not bind other IL-8-related peptides such as 125I-labeled NAP-2, 125I-labeled GRO, or 125I-labeled PF4. Furthermore, bound 125I-labeled IL-8 was only displaced by unlabeled IL-8 but not by unlabeled NAP-2, GROα, or PF4. Consistent with this observation, stably transfected CCL 39 cells expressing F3R cDNA mobilized Ca2+ only in response to IL-8. We conclude that F3R cDNA encodes a functional IL-8 receptor isotype with strict ligand binding specificity for IL-8, that rabbit neutrophils do not bind human GROα, and it is suggested that rabbit neutrophils contain in addition to the F3R protein another IL-8 receptor isotype with broad ligand specificity or a distinct NAP-2 receptor.

Original languageEnglish (US)
Pages (from-to)2496-2500
Number of pages5
JournalJournal of Immunology
Volume152
Issue number5
StatePublished - Mar 1 1994

Fingerprint

Interleukin-8 Receptors
Interleukin-8
Neutrophils
Rabbits
Ligands
Platelet Factor 4
Complementary DNA
COS Cells
Neutrophil Activation
Peptides
Peptide Receptors
Chemokines
Cricetinae
Haplorhini
connective tissue-activating peptide
Melanoma
Fibroblasts

ASJC Scopus subject areas

  • Immunology

Cite this

Thomas, K. M., Taylor, L., Prado, G., Romero, J., Moser, B., Car, B., ... Navarro, J. (1994). Functional and ligand binding specificity of the rabbit neutrophil IL-8 receptor. Journal of Immunology, 152(5), 2496-2500.

Functional and ligand binding specificity of the rabbit neutrophil IL-8 receptor. / Thomas, Kathleen M.; Taylor, Linda; Prado, Gregory; Romero, Jose; Moser, Bernhard; Car, Bruce; Walz, Alfred; Baggiolini, Marco; Navarro, Javier.

In: Journal of Immunology, Vol. 152, No. 5, 01.03.1994, p. 2496-2500.

Research output: Contribution to journalArticle

Thomas, KM, Taylor, L, Prado, G, Romero, J, Moser, B, Car, B, Walz, A, Baggiolini, M & Navarro, J 1994, 'Functional and ligand binding specificity of the rabbit neutrophil IL-8 receptor', Journal of Immunology, vol. 152, no. 5, pp. 2496-2500.
Thomas KM, Taylor L, Prado G, Romero J, Moser B, Car B et al. Functional and ligand binding specificity of the rabbit neutrophil IL-8 receptor. Journal of Immunology. 1994 Mar 1;152(5):2496-2500.
Thomas, Kathleen M. ; Taylor, Linda ; Prado, Gregory ; Romero, Jose ; Moser, Bernhard ; Car, Bruce ; Walz, Alfred ; Baggiolini, Marco ; Navarro, Javier. / Functional and ligand binding specificity of the rabbit neutrophil IL-8 receptor. In: Journal of Immunology. 1994 ; Vol. 152, No. 5. pp. 2496-2500.
@article{94e7bb99f0af4f51a8280a1464b61f8b,
title = "Functional and ligand binding specificity of the rabbit neutrophil IL-8 receptor",
abstract = "IL-8 mediates migration and activation of neutrophils. This study describes the functional and ligand binding specificity of the human intercrine peptides IL-8, neutrophil-activating peptide 2 (NAP-2), melanoma growth stimulatory activity (GRO), and platelet factor 4 (PF4) to rabbit neutrophils and mammalian cell lines transfected with rabbit IL-8 receptor cDNA (F3R). Rabbit neutrophil membranes bound 125I-labeled IL-8 and 125I-labeled NAP-2 but did not bind 125I-labeled GRO or 125I- labeled PF4. Rabbit neutrophils mobilized intracellular Ca2+ in response to IL-8 and NAP-2 but not to GRO or PF4. Monkey kidney cells (COS-7) and hamster lung fibroblasts (CCL-39) were transiently and stably transfected with the rabbit neutrophil IL-8 receptor F3R cDNA. COS-7 cells transfected with F3R cDNA bound 125I-labeled IL-8 but did not bind other IL-8-related peptides such as 125I-labeled NAP-2, 125I-labeled GRO, or 125I-labeled PF4. Furthermore, bound 125I-labeled IL-8 was only displaced by unlabeled IL-8 but not by unlabeled NAP-2, GROα, or PF4. Consistent with this observation, stably transfected CCL 39 cells expressing F3R cDNA mobilized Ca2+ only in response to IL-8. We conclude that F3R cDNA encodes a functional IL-8 receptor isotype with strict ligand binding specificity for IL-8, that rabbit neutrophils do not bind human GROα, and it is suggested that rabbit neutrophils contain in addition to the F3R protein another IL-8 receptor isotype with broad ligand specificity or a distinct NAP-2 receptor.",
author = "Thomas, {Kathleen M.} and Linda Taylor and Gregory Prado and Jose Romero and Bernhard Moser and Bruce Car and Alfred Walz and Marco Baggiolini and Javier Navarro",
year = "1994",
month = "3",
day = "1",
language = "English (US)",
volume = "152",
pages = "2496--2500",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "5",

}

TY - JOUR

T1 - Functional and ligand binding specificity of the rabbit neutrophil IL-8 receptor

AU - Thomas, Kathleen M.

AU - Taylor, Linda

AU - Prado, Gregory

AU - Romero, Jose

AU - Moser, Bernhard

AU - Car, Bruce

AU - Walz, Alfred

AU - Baggiolini, Marco

AU - Navarro, Javier

PY - 1994/3/1

Y1 - 1994/3/1

N2 - IL-8 mediates migration and activation of neutrophils. This study describes the functional and ligand binding specificity of the human intercrine peptides IL-8, neutrophil-activating peptide 2 (NAP-2), melanoma growth stimulatory activity (GRO), and platelet factor 4 (PF4) to rabbit neutrophils and mammalian cell lines transfected with rabbit IL-8 receptor cDNA (F3R). Rabbit neutrophil membranes bound 125I-labeled IL-8 and 125I-labeled NAP-2 but did not bind 125I-labeled GRO or 125I- labeled PF4. Rabbit neutrophils mobilized intracellular Ca2+ in response to IL-8 and NAP-2 but not to GRO or PF4. Monkey kidney cells (COS-7) and hamster lung fibroblasts (CCL-39) were transiently and stably transfected with the rabbit neutrophil IL-8 receptor F3R cDNA. COS-7 cells transfected with F3R cDNA bound 125I-labeled IL-8 but did not bind other IL-8-related peptides such as 125I-labeled NAP-2, 125I-labeled GRO, or 125I-labeled PF4. Furthermore, bound 125I-labeled IL-8 was only displaced by unlabeled IL-8 but not by unlabeled NAP-2, GROα, or PF4. Consistent with this observation, stably transfected CCL 39 cells expressing F3R cDNA mobilized Ca2+ only in response to IL-8. We conclude that F3R cDNA encodes a functional IL-8 receptor isotype with strict ligand binding specificity for IL-8, that rabbit neutrophils do not bind human GROα, and it is suggested that rabbit neutrophils contain in addition to the F3R protein another IL-8 receptor isotype with broad ligand specificity or a distinct NAP-2 receptor.

AB - IL-8 mediates migration and activation of neutrophils. This study describes the functional and ligand binding specificity of the human intercrine peptides IL-8, neutrophil-activating peptide 2 (NAP-2), melanoma growth stimulatory activity (GRO), and platelet factor 4 (PF4) to rabbit neutrophils and mammalian cell lines transfected with rabbit IL-8 receptor cDNA (F3R). Rabbit neutrophil membranes bound 125I-labeled IL-8 and 125I-labeled NAP-2 but did not bind 125I-labeled GRO or 125I- labeled PF4. Rabbit neutrophils mobilized intracellular Ca2+ in response to IL-8 and NAP-2 but not to GRO or PF4. Monkey kidney cells (COS-7) and hamster lung fibroblasts (CCL-39) were transiently and stably transfected with the rabbit neutrophil IL-8 receptor F3R cDNA. COS-7 cells transfected with F3R cDNA bound 125I-labeled IL-8 but did not bind other IL-8-related peptides such as 125I-labeled NAP-2, 125I-labeled GRO, or 125I-labeled PF4. Furthermore, bound 125I-labeled IL-8 was only displaced by unlabeled IL-8 but not by unlabeled NAP-2, GROα, or PF4. Consistent with this observation, stably transfected CCL 39 cells expressing F3R cDNA mobilized Ca2+ only in response to IL-8. We conclude that F3R cDNA encodes a functional IL-8 receptor isotype with strict ligand binding specificity for IL-8, that rabbit neutrophils do not bind human GROα, and it is suggested that rabbit neutrophils contain in addition to the F3R protein another IL-8 receptor isotype with broad ligand specificity or a distinct NAP-2 receptor.

UR - http://www.scopus.com/inward/record.url?scp=0028224185&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028224185&partnerID=8YFLogxK

M3 - Article

C2 - 8133060

AN - SCOPUS:0028224185

VL - 152

SP - 2496

EP - 2500

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 5

ER -