Functional characterization of naturally occurring variants of human hepatitis B virus containing the core internal deletion mutation

Thomas Ta Tung Yuan, Min Hui Lin, Suimin Qiu, Chiaho Shih

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Naturally occurring variants of human hepatitis B virus (HBV) containing the core internal deletion (CID) mutation have been found frequently in HBV carriers worldwide. Despite numerous sequence analysis reports of CID variants in patients, in the past decade, CID variants have not been characterized functionally, and thus their biological significance to HBV infection remains unclear. We report here two different CID variants identified from two patients that are replication defective, most likely due to the absence of detectable core protein. In addition, we were unable to detect the presence of the precore protein and e antigen from CID variants. However, the production of polymerase appeared to be normal. The replication defect of the CID variants can be rescued in trans by complementation with wild-type core protein. The rescued CID variant particles, which utilize the wild-type core protein, presumably are enveloped properly since they can be secreted into the medium and hand at a position similar to that of mature wild-type Dane particles, as determined by gradient centrifugation analysis. Our results also provide an explanation for the association of CID variants with helper or wild-type HBV in nature. The significance of CID variants in HBV infection and pathogenesis is discussed.

Original languageEnglish (US)
Pages (from-to)2168-2176
Number of pages9
JournalJournal of Virology
Volume72
Issue number3
StatePublished - Mar 1998

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Hepatitis B virus
Sequence Deletion
Virus Diseases
proteins
Proteins
Hepatitis B e Antigens
infection
centrifugation
Centrifugation
hands
pathogenesis
sequence analysis
Sequence Analysis
sequence deletion
antigens
Hand

ASJC Scopus subject areas

  • Immunology

Cite this

Functional characterization of naturally occurring variants of human hepatitis B virus containing the core internal deletion mutation. / Yuan, Thomas Ta Tung; Lin, Min Hui; Qiu, Suimin; Shih, Chiaho.

In: Journal of Virology, Vol. 72, No. 3, 03.1998, p. 2168-2176.

Research output: Contribution to journalArticle

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abstract = "Naturally occurring variants of human hepatitis B virus (HBV) containing the core internal deletion (CID) mutation have been found frequently in HBV carriers worldwide. Despite numerous sequence analysis reports of CID variants in patients, in the past decade, CID variants have not been characterized functionally, and thus their biological significance to HBV infection remains unclear. We report here two different CID variants identified from two patients that are replication defective, most likely due to the absence of detectable core protein. In addition, we were unable to detect the presence of the precore protein and e antigen from CID variants. However, the production of polymerase appeared to be normal. The replication defect of the CID variants can be rescued in trans by complementation with wild-type core protein. The rescued CID variant particles, which utilize the wild-type core protein, presumably are enveloped properly since they can be secreted into the medium and hand at a position similar to that of mature wild-type Dane particles, as determined by gradient centrifugation analysis. Our results also provide an explanation for the association of CID variants with helper or wild-type HBV in nature. The significance of CID variants in HBV infection and pathogenesis is discussed.",
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