Functional classification of protein toxins as a basis for bioinformatic screening

Surendra S. Negi, Catherine H. Schein, Gregory S. Ladics, Henry Mirsky, Peter Chang, Jean Baptiste Rascle, John Kough, Lieven Sterck, Sabitha Papineni, Joseph M. Jez, Lucilia Pereira Mouriès, Werner Braun

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Proteins are fundamental to life and exhibit a wide diversity of activities, some of which are toxic. Therefore, assessing whether a specific protein is safe for consumption in foods and feeds is critical. Simple BLAST searches may reveal homology to a known toxin, when in fact the protein may pose no real danger. Another challenge to answer this question is the lack of curated databases with a representative set of experimentally validated toxins. Here we have systematically analyzed over 10,000 manually curated toxin sequences using sequence clustering, network analysis, and protein domain classification. We also developed a functional sequence signature method to distinguish toxic from non-toxic proteins. The current database, combined with motif analysis, can be used by researchers and regulators in a hazard screening capacity to assess the potential of a protein to be toxic at early stages of development. Identifying key signatures of toxicity can also aid in redesigning proteins, so as to maintain their desirable functions while reducing the risk of potential health hazards.

Original languageEnglish (US)
Article number13940
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Computational Biology
Poisons
Proteins
Databases
Cluster Analysis
Research Personnel
Food
Health

ASJC Scopus subject areas

  • General

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Negi, S. S., Schein, C. H., Ladics, G. S., Mirsky, H., Chang, P., Rascle, J. B., ... Braun, W. (2017). Functional classification of protein toxins as a basis for bioinformatic screening. Scientific Reports, 7(1), [13940]. https://doi.org/10.1038/s41598-017-13957-1

Functional classification of protein toxins as a basis for bioinformatic screening. / Negi, Surendra S.; Schein, Catherine H.; Ladics, Gregory S.; Mirsky, Henry; Chang, Peter; Rascle, Jean Baptiste; Kough, John; Sterck, Lieven; Papineni, Sabitha; Jez, Joseph M.; Pereira Mouriès, Lucilia; Braun, Werner.

In: Scientific Reports, Vol. 7, No. 1, 13940, 01.12.2017.

Research output: Contribution to journalArticle

Negi, SS, Schein, CH, Ladics, GS, Mirsky, H, Chang, P, Rascle, JB, Kough, J, Sterck, L, Papineni, S, Jez, JM, Pereira Mouriès, L & Braun, W 2017, 'Functional classification of protein toxins as a basis for bioinformatic screening', Scientific Reports, vol. 7, no. 1, 13940. https://doi.org/10.1038/s41598-017-13957-1
Negi SS, Schein CH, Ladics GS, Mirsky H, Chang P, Rascle JB et al. Functional classification of protein toxins as a basis for bioinformatic screening. Scientific Reports. 2017 Dec 1;7(1). 13940. https://doi.org/10.1038/s41598-017-13957-1
Negi, Surendra S. ; Schein, Catherine H. ; Ladics, Gregory S. ; Mirsky, Henry ; Chang, Peter ; Rascle, Jean Baptiste ; Kough, John ; Sterck, Lieven ; Papineni, Sabitha ; Jez, Joseph M. ; Pereira Mouriès, Lucilia ; Braun, Werner. / Functional classification of protein toxins as a basis for bioinformatic screening. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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