Functional dissection of the human TNRC6 (GW182-related) family of proteins

David Baillat, Ramin Shiekhattar

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Argonaute (Ago) proteins through their association with small RNAs perform a critical function in the effector step of RNA interference. The TNRC6 (trinucleotide repeat containing 6) family of proteins have been shown to stably associate with Agos in mammalian cells. Here, we describe the isolation and functional characterization of TNRC6B- and TNRC6C-containing complexes. We show that TNRC6B and TNRC6C proteins associate with all four human Agos which are already loaded with microRNAs. Detailed domain analysis of TNRC6B protein indicated that distinct domains of the protein are required for Ago binding and P-body localization. Functional analysis using reporter constructs responsive to TNRC6B tethered through an MS2-binding domain indicates that neither the Ago-binding nor the P-body localization domains are required for translational silencing. In contrast, the C-terminal domain containing the RNA recognition motif plays a critical role in the silencing mediated by the TNRC6B protein.

Original languageEnglish (US)
Pages (from-to)4144-4155
Number of pages12
JournalMolecular and Cellular Biology
Volume29
Issue number15
DOIs
StatePublished - Aug 2009
Externally publishedYes

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Trinucleotide Repeats
Dissection
Argonaute Proteins
Proteins
RNA Interference
MicroRNAs
RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Functional dissection of the human TNRC6 (GW182-related) family of proteins. / Baillat, David; Shiekhattar, Ramin.

In: Molecular and Cellular Biology, Vol. 29, No. 15, 08.2009, p. 4144-4155.

Research output: Contribution to journalArticle

Baillat, David ; Shiekhattar, Ramin. / Functional dissection of the human TNRC6 (GW182-related) family of proteins. In: Molecular and Cellular Biology. 2009 ; Vol. 29, No. 15. pp. 4144-4155.
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