Functional regulation of oestrogen receptor pathway by the dynein light chain 1

Suresh K. Rayala, Petra den Hollander, Seetharaman Balasenthil, Zhibo Yang, Russell R. Broaddus, Rakesh Kumar

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

Overexpression and phosphorylation of dynein light chain 1 (DLC1) have been shown to promote the growth of breast cancer cells. However, the role of DLC1 in the action of the oestrogen receptor (ER) remains unknown. Here, we found that oestrogen induces the transcription and expression of DLC1. DLC1 facilitated oestrogen-induced ER transactivation and anchorage-independent growth of breast cancer cells. We show that DLC1 interacts with ER, and such interaction is required for the transactivation-promoting activity of DLC1. Further, DLC1 expression led to enhanced recruitment of the DLC1-ER complex to the ER-target gene chromatin. Conversely, DLC1 down-regulation compromised the ER-transactivation activity and also its nuclear accumulation, suggesting a potential chaperone-like activity of DLC1 in the nuclear translocation of ER. Together, these data define an unexpected upregulation of DLC1 by oestrogen and a previously unrecognized DLC1-ER interaction in supporting and amplifying ER-initiated cellular responses in breast cancer cells.

Original languageEnglish (US)
Pages (from-to)538-544
Number of pages7
JournalEMBO reports
Volume6
Issue number6
DOIs
StatePublished - Jun 2005

Keywords

  • Breast cancer cells
  • Dynein
  • Gene expression
  • Oestrogen receptor
  • Signalling

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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