Functional T cell subsets contribute differentially to HIV peptide-specific responses within infected individuals: Correlation of these functional T cell subsets with markers of disease progression

Yoav Peretz, Michel L. Ndongala, Salix Boulet, Mohamed R. Boulassel, Danielle Rouleau, Pierre Côté, Danièle Longpré, Jean Pierre Routy, Julian Falutz, Cécile Tremblay, Christos M. Tsoukas, Rafick P. Sékaly, Nicole F. Bernard

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Using a dual color ELISPOT assay able to detect HIV-specific IFN-γ, IL-2 and dual IFN-γ/IL-2 secreting lymphocytes we screened for HIV peptide-specific responses directed against the entire HIV proteome in two groups of untreated HIV-infected individuals, slow progressors (SP) and progressors. We found that the three functional lymphocyte subsets contributed differentially to individual HIV peptide-specific responses within a study subject. Among the identified stimulatory peptides, a higher proportion induced dual IFN-γ/IL-2 secretion in SP than progressors. While the magnitude of single IFN-γ secreting lymphocytes is similar between groups, the magnitude of peptide-specific dual IFN-γ/IL-2 secreting lymphocytes is significantly more intense in SP. Neither single nor total IFN-γ secreting cell magnitude and breadth measurements correlated with CD4 cell count or viral load whereas both parameters of dual IFN-γ/IL-2 secreting responses correlated positively with CD4 counts and negatively with viremia.

Original languageEnglish (US)
Pages (from-to)57-68
Number of pages12
JournalClinical Immunology
Volume124
Issue number1
DOIs
StatePublished - Jul 2007

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Keywords

  • AIDS
  • Cellular immunology
  • Disease progression
  • Dual color ELISPOT
  • Functional subsets
  • HIV
  • Long term non progressors
  • T cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Peretz, Y., Ndongala, M. L., Boulet, S., Boulassel, M. R., Rouleau, D., Côté, P., Longpré, D., Routy, J. P., Falutz, J., Tremblay, C., Tsoukas, C. M., Sékaly, R. P., & Bernard, N. F. (2007). Functional T cell subsets contribute differentially to HIV peptide-specific responses within infected individuals: Correlation of these functional T cell subsets with markers of disease progression. Clinical Immunology, 124(1), 57-68. https://doi.org/10.1016/j.clim.2007.04.004