Functions of selected biochemical systems from the exercised trained dog heart

L. A. Sordahl, G. K. Asimakis, R. T. Dowell, H. L. Stone

    Research output: Chapter in Book/Report/Conference proceedingChapter

    24 Citations (Scopus)

    Abstract

    Mitochondria and sarcoplasmic reticulum (SR) fractions were isolated from exercised trained (E T) and sedentary control dog hearts. Measurements of mitochondrial respiratory functions indicated no changes in energy producing (ATP synthesis) capacity in mitochondria from E T compared to control dog hearts. However, the ability of isolated mitochondria from E T hearts to retain accumulated calcium was markedly decreased compared to controls. Inhibition of mitochondrial rates of calcium uptake with the inhibitor, ruthenium red, revealed fewer binding and/or transport sites in mitochondrial membranes from exercised trained heart preparations. ATP dependent binding (-oxalate) and uptake (+ oxalate) of calcium by SR preparations from E T hearts were unchanged compared to controls. In contrast, significant differences in the rates of release of bound calcium were found in SR isolated from E T hearts. Total myocardial protein, nucleic acids, and connective tissue levels were unchanged in E T hearts compared to controls. The results suggest subtle changes are occurring in the energy utilizing mechanism(s) involving calcium transport of the myocardial cell during exercise training. These changes may be related to alterations in the performance of the exercised trained heart.

    Original languageEnglish (US)
    Title of host publicationJ.APPL.PHYSIOL.RESPIR.ENVIRON.EXERCISE PHYSIOL.
    Pages426-431
    Number of pages6
    Volume42
    Edition3
    StatePublished - 1977

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    Dogs
    Sarcoplasmic Reticulum
    Calcium
    Mitochondria
    Adenosine Triphosphate
    Ruthenium Red
    Calcium Oxalate
    Oxalates
    Mitochondrial Membranes
    Connective Tissue
    Nucleic Acids
    Exercise
    Proteins

    ASJC Scopus subject areas

    • Medicine(all)

    Cite this

    Sordahl, L. A., Asimakis, G. K., Dowell, R. T., & Stone, H. L. (1977). Functions of selected biochemical systems from the exercised trained dog heart. In J.APPL.PHYSIOL.RESPIR.ENVIRON.EXERCISE PHYSIOL. (3 ed., Vol. 42, pp. 426-431)

    Functions of selected biochemical systems from the exercised trained dog heart. / Sordahl, L. A.; Asimakis, G. K.; Dowell, R. T.; Stone, H. L.

    J.APPL.PHYSIOL.RESPIR.ENVIRON.EXERCISE PHYSIOL.. Vol. 42 3. ed. 1977. p. 426-431.

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Sordahl, LA, Asimakis, GK, Dowell, RT & Stone, HL 1977, Functions of selected biochemical systems from the exercised trained dog heart. in J.APPL.PHYSIOL.RESPIR.ENVIRON.EXERCISE PHYSIOL.. 3 edn, vol. 42, pp. 426-431.
    Sordahl LA, Asimakis GK, Dowell RT, Stone HL. Functions of selected biochemical systems from the exercised trained dog heart. In J.APPL.PHYSIOL.RESPIR.ENVIRON.EXERCISE PHYSIOL.. 3 ed. Vol. 42. 1977. p. 426-431
    Sordahl, L. A. ; Asimakis, G. K. ; Dowell, R. T. ; Stone, H. L. / Functions of selected biochemical systems from the exercised trained dog heart. J.APPL.PHYSIOL.RESPIR.ENVIRON.EXERCISE PHYSIOL.. Vol. 42 3. ed. 1977. pp. 426-431
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