Fungal Secretome Analysis via PepSAVI-MS: Identification of the Bioactive Peptide KP4 from Ustilago maydis

Christine L. Kirkpatrick, Nicole C. Parsley, Tessa E. Bartges, Madeline E. Cooke, Wilaysha S. Evans, Lilian R. Heil, Thomas Smith, Leslie M. Hicks

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Fungal secondary metabolites represent a rich and largely untapped source for bioactive molecules, including peptides with substantial structural diversity and pharmacological potential. As methods proceed to take a deep dive into fungal genomes, complimentary methods to identify bioactive components are required to keep pace with the expanding fungal repertoire. We developed PepSAVI-MS to expedite the search for natural product bioactive peptides and herein demonstrate proof-of-principle applicability of the pipeline for the discovery of bioactive peptides from fungal secretomes via identification of the antifungal killer toxin KP4 from Ustilago maydis P4. This work opens the door to investigating microbial secretomes with a new lens, and could have broad applications across human health, agriculture, and food safety.

Original languageEnglish (US)
Pages (from-to)859-865
Number of pages7
JournalJournal of the American Society for Mass Spectrometry
Volume29
Issue number5
DOIs
StatePublished - May 1 2018

Keywords

  • Antimicrobial peptides (AMPs)
  • Bioactive peptides
  • Fungal secretomes
  • Mass spectrometry
  • Natural products
  • Peptidomics

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

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