Fungal virus capsids, cytoplasmic compartments for the replication of double-stranded RNA, formed as icosahedral shells of asymmetric gag dimers

R. Holland Cheng, Jose R. Caston, Guo Ji Wang, Fei Gu, Thomas Smith, Timothy S. Baker, Robert F. Bozarth, Benes L. Trus, Naiqian Cheng, Reed B. Wickner, Alasdair C. Steven

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

The primary functions of most virus capsids are to protect the viral genome in the extra-cellular milieu and deliver it to the host. In contrast, the capsids of fungal viruses, like the cores of all other known double stranded RNA viruses, are not involved in host recognition but do shield their genomes, and they also carry out transcription and replication. Nascent (+) strands are extruded from transcribing virions. The capsids of the yeast virus L-A are composed of Gag (capsid protein; 76 kDa), with a few molecules of Gag-Pol (170 kDa). Analysis of these 420 Å diameter shells and those of the fungal P4 virus by cryo-electron microscopy and image reconstruction shows that they share the same novel icosahedral structure. Both capsids consist of 60 equivalent Gag dimers, whose two subunits occupy non-equivalent bonding environments. Stoichiometry data on other double-stranded RNA viruses indicate that the 120-subunit structure is widespread, implying that this molecular architecture has features that are particularly favorable to the design of a capsid that is also a biosynthetic compartment.

Original languageEnglish (US)
Pages (from-to)255-258
Number of pages4
JournalJournal of Molecular Biology
Volume244
Issue number3
DOIs
StatePublished - 1994
Externally publishedYes

Fingerprint

Double-Stranded RNA
Capsid
RNA Viruses
Viruses
Cryoelectron Microscopy
gag Gene Products
Computer-Assisted Image Processing
Viral Genome
Capsid Proteins
Virion
Yeasts
Fungal Viruses
Genome

Keywords

  • Cryo-electron microscopy
  • Double-stranded RNA
  • Quasi-equivalence
  • Three-dimensional image reconstruction
  • Virus capsid protein

ASJC Scopus subject areas

  • Virology

Cite this

Fungal virus capsids, cytoplasmic compartments for the replication of double-stranded RNA, formed as icosahedral shells of asymmetric gag dimers. / Cheng, R. Holland; Caston, Jose R.; Wang, Guo Ji; Gu, Fei; Smith, Thomas; Baker, Timothy S.; Bozarth, Robert F.; Trus, Benes L.; Cheng, Naiqian; Wickner, Reed B.; Steven, Alasdair C.

In: Journal of Molecular Biology, Vol. 244, No. 3, 1994, p. 255-258.

Research output: Contribution to journalArticle

Cheng, RH, Caston, JR, Wang, GJ, Gu, F, Smith, T, Baker, TS, Bozarth, RF, Trus, BL, Cheng, N, Wickner, RB & Steven, AC 1994, 'Fungal virus capsids, cytoplasmic compartments for the replication of double-stranded RNA, formed as icosahedral shells of asymmetric gag dimers', Journal of Molecular Biology, vol. 244, no. 3, pp. 255-258. https://doi.org/10.1006/jmbi.1994.1726
Cheng, R. Holland ; Caston, Jose R. ; Wang, Guo Ji ; Gu, Fei ; Smith, Thomas ; Baker, Timothy S. ; Bozarth, Robert F. ; Trus, Benes L. ; Cheng, Naiqian ; Wickner, Reed B. ; Steven, Alasdair C. / Fungal virus capsids, cytoplasmic compartments for the replication of double-stranded RNA, formed as icosahedral shells of asymmetric gag dimers. In: Journal of Molecular Biology. 1994 ; Vol. 244, No. 3. pp. 255-258.
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