Further evaluation of a mutagen-attenuated Rift Valley fever vaccine in sheep

J. C. Morrill, L. Carpenter, D. Taylor, H. H. Ramsburg, J. Quance, C. J. Peters

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

A previous study demonstrated that a mutagen-attenuated Rift Valley fever virus (RVFV) vaccine, RVF MP-12, was immunogenic and non-abortogenic when ewes, 90 110 days pregnant, were inoculated with 5 × 105 plaque-forming units (p.f.u.) of the virus strain. The ewes delivered live, healthy lambs that had no neutralizing antibody to RVFV until after they had ingested colostrum. To assess further the safety and protective capability of this candidate vaccine, six pregnant ewes were inoculated with 5 × 103 p.f.u. of RVF MP-12 and challenged with 5 × 105 p.f.u. of virulent ZH-501 strain of RVFV 30 days later. No viraemia was detected after vaccination or challenge and all six ewes delivered live, healthy lambs. Those lambs tested before their nursing did not have neutralizing antibody to RVFV but quickly acquired antibody titres of 1:320 to ≥ 1:10 240 after ingesting colostrum. To test the safety of the RVF MP-12 immunogen in neonates, lambs ≤ 7 days old, born to unvaccinated ewes, were inoculated with 5 × 105 p.f.u. of RVF MP-12. With the exception of brief pyrexia in 18 of 26 lambs, and a transient low-titred viraemia in 16 of 26 lambs after inoculation, no untoward effects were observed. Serum-neutralizing antibody to RVFV was detected 5-7 days after inoculation. Lambs vaccinated with either 5 × 105 or 5 × 103 p.f.u. of RVF MP-12 were protected against virulent RVFV challenge at 14 days postvaccination.

Original languageEnglish (US)
Pages (from-to)35-41
Number of pages7
JournalVaccine
Volume9
Issue number1
DOIs
StatePublished - Jan 1991

Keywords

  • Rift Valley fever
  • attenuated
  • mutagen
  • sheep
  • vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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