Further investigations of mutagenic cholesterol preparations

G. A.S. Ansari, R. D. Walker, V. B. Smart, L. L. Smith

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

The previously demonstrated mutagenicity of naturally air-aged (autoxidized) USP cholesterol in test strains of Salmonella typhimurium has been confirmed. In contrast, autoxidized brassicasterol, 7-dehydrocholesterol, ergosterol, lanosterol, sitosterol and stigmasterol were non-mutagenic in the same assay, and 38 individual cholesterol derivatives, including many cholesterol autoxidation products, were also non-mutagenic. The mutagenic species from mutagenic cholesterol preparations were shown to be neutral steroids that are very much more polar than the cholesterol autoxidation products so far identified. High-performance liquid chromatography resolved mutagenic cholesterol preparations into several mutagenic and non-mutagenic fractions. The chemical nature of the mutagens is suggested as involving oxidation of the sterol B-ring and of the side-chain.

Original languageEnglish (US)
Pages (from-to)35-41
Number of pages7
JournalFood and Chemical Toxicology
Volume20
Issue number1
DOIs
StatePublished - 1982

ASJC Scopus subject areas

  • Food Science
  • Toxicology

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