Further investigations of mutagenic cholesterol preparations

G. A.S. Ansari; R. D. Walker; V. B. Smart; L. L. Smith

doi:10.1016/S0278-6915(82)80006-9

Further investigations of mutagenic cholesterol preparations

G. A.S. Ansari
, R. D. Walker
, V. B. Smart
, L. L. Smith

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

The previously demonstrated mutagenicity of naturally air-aged (autoxidized) USP cholesterol in test strains of Salmonella typhimurium has been confirmed. In contrast, autoxidized brassicasterol, 7-dehydrocholesterol, ergosterol, lanosterol, sitosterol and stigmasterol were non-mutagenic in the same assay, and 38 individual cholesterol derivatives, including many cholesterol autoxidation products, were also non-mutagenic. The mutagenic species from mutagenic cholesterol preparations were shown to be neutral steroids that are very much more polar than the cholesterol autoxidation products so far identified. High-performance liquid chromatography resolved mutagenic cholesterol preparations into several mutagenic and non-mutagenic fractions. The chemical nature of the mutagens is suggested as involving oxidation of the sterol B-ring and of the side-chain.

Original language	English (US)
Pages (from-to)	35-41
Number of pages	7
Journal	Food and Chemical Toxicology
Volume	20
Issue number	1
DOIs	https://doi.org/10.1016/S0278-6915(82)80006-9
State	Published - 1982
Externally published	Yes

ASJC Scopus subject areas

Food Science
Toxicology

Access to Document

10.1016/S0278-6915(82)80006-9

Cite this

@article{77d33bda7d85431387cff7188fa2c062,

title = "Further investigations of mutagenic cholesterol preparations",

abstract = "The previously demonstrated mutagenicity of naturally air-aged (autoxidized) USP cholesterol in test strains of Salmonella typhimurium has been confirmed. In contrast, autoxidized brassicasterol, 7-dehydrocholesterol, ergosterol, lanosterol, sitosterol and stigmasterol were non-mutagenic in the same assay, and 38 individual cholesterol derivatives, including many cholesterol autoxidation products, were also non-mutagenic. The mutagenic species from mutagenic cholesterol preparations were shown to be neutral steroids that are very much more polar than the cholesterol autoxidation products so far identified. High-performance liquid chromatography resolved mutagenic cholesterol preparations into several mutagenic and non-mutagenic fractions. The chemical nature of the mutagens is suggested as involving oxidation of the sterol B-ring and of the side-chain.",

author = "Ansari, \{G. A.S.\} and Walker, \{R. D.\} and Smart, \{V. B.\} and Smith, \{L. L.\}",

note = "Funding Information: Acknorv/edgemenrs-The Clark in conducting reported here is gratefully Ames. University of California. us with the Sa/monr//a rJp/rimrrrium study was financially supported the US Public Health Service.",

year = "1982",

doi = "10.1016/S0278-6915(82)80006-9",

language = "English (US)",

volume = "20",

pages = "35--41",

journal = "Food and Chemical Toxicology",

issn = "0278-6915",

publisher = "Elsevier Ltd",

number = "1",

}

TY - JOUR

T1 - Further investigations of mutagenic cholesterol preparations

AU - Ansari, G. A.S.

AU - Walker, R. D.

AU - Smart, V. B.

AU - Smith, L. L.

N1 - Funding Information: Acknorv/edgemenrs-The Clark in conducting reported here is gratefully Ames. University of California. us with the Sa/monr//a rJp/rimrrrium study was financially supported the US Public Health Service.

PY - 1982

Y1 - 1982

N2 - The previously demonstrated mutagenicity of naturally air-aged (autoxidized) USP cholesterol in test strains of Salmonella typhimurium has been confirmed. In contrast, autoxidized brassicasterol, 7-dehydrocholesterol, ergosterol, lanosterol, sitosterol and stigmasterol were non-mutagenic in the same assay, and 38 individual cholesterol derivatives, including many cholesterol autoxidation products, were also non-mutagenic. The mutagenic species from mutagenic cholesterol preparations were shown to be neutral steroids that are very much more polar than the cholesterol autoxidation products so far identified. High-performance liquid chromatography resolved mutagenic cholesterol preparations into several mutagenic and non-mutagenic fractions. The chemical nature of the mutagens is suggested as involving oxidation of the sterol B-ring and of the side-chain.

AB - The previously demonstrated mutagenicity of naturally air-aged (autoxidized) USP cholesterol in test strains of Salmonella typhimurium has been confirmed. In contrast, autoxidized brassicasterol, 7-dehydrocholesterol, ergosterol, lanosterol, sitosterol and stigmasterol were non-mutagenic in the same assay, and 38 individual cholesterol derivatives, including many cholesterol autoxidation products, were also non-mutagenic. The mutagenic species from mutagenic cholesterol preparations were shown to be neutral steroids that are very much more polar than the cholesterol autoxidation products so far identified. High-performance liquid chromatography resolved mutagenic cholesterol preparations into several mutagenic and non-mutagenic fractions. The chemical nature of the mutagens is suggested as involving oxidation of the sterol B-ring and of the side-chain.

UR - https://www.scopus.com/pages/publications/0019989378

UR - https://www.scopus.com/pages/publications/0019989378#tab=citedBy

U2 - 10.1016/S0278-6915(82)80006-9

DO - 10.1016/S0278-6915(82)80006-9

M3 - Article

C2 - 7040182

AN - SCOPUS:0019989378

SN - 0278-6915

VL - 20

SP - 35

EP - 41

JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

IS - 1

ER -

Further investigations of mutagenic cholesterol preparations

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this