Further studies on the radiation-induced expression of a tumor-specific antigen in human cell hybrids

C. Sun, J. L. Redpath, M. Colman, E. J. Stanbridge

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The neoplastic transformation of human cell hybrids (HeLa X skin fibroblasts) is accompanied by the expression of a cell surface protein for which monoclonal antibodies have been raised. The γ-radiation-induced neoplastic transformation of these cells has been studied where the expression of this cell surface protein, as detected by immunoperoxidase staining, has been used as an end point. The yield of foci of positively staining cells has been shown to increase with increasing time postirradiation at which the assay is done and decrease with increasing density of viable cells plated postirradiation. The time of plating postirradiation is also an important parameter with transformation frequencies increasing over the first 6 h of postirradiation holding at confluence, followed by a gradual decrease.

Original languageEnglish
Pages (from-to)84-93
Number of pages10
JournalRadiation Research
Volume114
Issue number1
StatePublished - 1988
Externally publishedYes

Fingerprint

Neoplastic Cell Transformation
Hybrid Cells
Neoplasm Antigens
antigens
Membrane Proteins
tumors
Radiation
Staining and Labeling
neoplasms
radiation
cells
surface proteins
staining
Fibroblasts
Cell Count
Monoclonal Antibodies
Skin
proteins
fibroblasts
antibodies

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Sun, C., Redpath, J. L., Colman, M., & Stanbridge, E. J. (1988). Further studies on the radiation-induced expression of a tumor-specific antigen in human cell hybrids. Radiation Research, 114(1), 84-93.

Further studies on the radiation-induced expression of a tumor-specific antigen in human cell hybrids. / Sun, C.; Redpath, J. L.; Colman, M.; Stanbridge, E. J.

In: Radiation Research, Vol. 114, No. 1, 1988, p. 84-93.

Research output: Contribution to journalArticle

Sun, C, Redpath, JL, Colman, M & Stanbridge, EJ 1988, 'Further studies on the radiation-induced expression of a tumor-specific antigen in human cell hybrids', Radiation Research, vol. 114, no. 1, pp. 84-93.
Sun, C. ; Redpath, J. L. ; Colman, M. ; Stanbridge, E. J. / Further studies on the radiation-induced expression of a tumor-specific antigen in human cell hybrids. In: Radiation Research. 1988 ; Vol. 114, No. 1. pp. 84-93.
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