Gain-of-function reporters for analysis of mrna 3´-end formation

Design and optimization

Natoya Peart, Eric Wagner

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    The concept of mRNA 3´-end formation as a static, minimally regulated housekeeping process has undergone a paradigm shift. Many recent studies have shown that accurate and efficient 3´-end formation of mRNA is highly regulated and that dysregulation of this process is a hallmark of several diseases. While there are many global analysis methods for monitoring altered mRNA processing, methods for investigating specific RNA 3´-end processing events in cells have not significantly changed. Here we describe a facile gain-of-function cellular reporter for the analysis of mRNA 3´-end formation as an alternative to approaches that are technically challenging or use radioactivity. We also offer suggestions for optimization of our approach and enhancement of its reproducibility.

    Original languageEnglish (US)
    Pages (from-to)137-140
    Number of pages4
    JournalBioTechniques
    Volume60
    Issue number3
    DOIs
    StatePublished - Mar 1 2016

    Fingerprint

    Messenger RNA
    RNA 3' End Processing
    Housekeeping
    Radioactivity
    Processing
    RNA
    Monitoring

    Keywords

    • 3´-end processing
    • Gain-of-function
    • Reporter assay

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Biotechnology

    Cite this

    Gain-of-function reporters for analysis of mrna 3´-end formation : Design and optimization. / Peart, Natoya; Wagner, Eric.

    In: BioTechniques, Vol. 60, No. 3, 01.03.2016, p. 137-140.

    Research output: Contribution to journalArticle

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