Gallotannin inhibits the expression of chemokines and inflammatory cytokines in A549 cells

Katalin Erdèlyi, Andrea Kiss, Edina Bakondi, Péter Bai, Csaba Szabo, Pál Gergely, Ferenc Erdodi, László Virág

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Tannins are plant-derived water-soluble polyphenols with wide-ranging biological activities. The mechanisms underlying the anti-inflammatory effect of tannins are not fully understood and may be the result of inhibition of poly(ADP-ribose) (PAR) glycohydrolase (PARG), the main catabolic enzyme of PAR metabolism. Therefore, we set out to investigate the mechanism of the anti-inflammatory effect of gallotannin (GT) in A549 cells with special regard to the role of poly(ADP-ribosyl)ation. Using an inflammation-focused low-density array and reverse transcription-polymerase chain reaction, we found that GT suppressed the expression of most cytokines and chemokines in cytokine-stimulated A549 cells, whereas the PARP inhibitor PJ-34 only inhibited few transcripts. Activation of the transcription factors, nuclear factor κB (NF-κB) and activator protein 1 (AP-1), was blocked by GT, whereas PJ-34 only suppressed NF-κB activation but not AP-1 activation. GT also inhibited IκB phosphorylation and nuclear translocation of NF-κB, but PJ-34 had no effect on these upstream events. In the AP-1 pathway, GT treatment, even in the absence of cytokines, caused maximal phosphorylation of c-Jun N-terminal kinase and c-Jun. GT also caused a low-level phosphorylation of p38, extracellular signal-regulated kinases 1 and 2, activating transcription factor2, and cAMP-response element-binding protein but inhibited cytokine-induced phosphorylation of these kinases and transcription factors. GT inhibited protein phosphatases 1 and 2A, which may explain the increased phosphorylation of mitogenactivated protein kinase and their substrates. GT exerted potent antioxidant effect but failed to cause PAR accumulation. In summary, the potent inhibitory effects of GT on the transcription of cytokine and chemokine genes are probably not related to PARG inhibition. Inhibition of AP-1 activation and upstream signaling events may be responsible for the effects of GT.

Original languageEnglish (US)
Pages (from-to)895-904
Number of pages10
JournalMolecular Pharmacology
Volume68
Issue number3
DOIs
StatePublished - Sep 2005
Externally publishedYes

Fingerprint

Hydrolyzable Tannins
Chemokines
Cytokines
Transcription Factor AP-1
Phosphorylation
Tannins
Anti-Inflammatory Agents
Transcription Factors
A549 Cells
Protein Phosphatase 1
Protein Phosphatase 2
Cyclic AMP Response Element-Binding Protein
Mitogen-Activated Protein Kinase 3
JNK Mitogen-Activated Protein Kinases
Glycoside Hydrolases
Mitogen-Activated Protein Kinase 1
Polyphenols
Adenosine Diphosphate
Protein Kinases
Reverse Transcription

ASJC Scopus subject areas

  • Pharmacology

Cite this

Gallotannin inhibits the expression of chemokines and inflammatory cytokines in A549 cells. / Erdèlyi, Katalin; Kiss, Andrea; Bakondi, Edina; Bai, Péter; Szabo, Csaba; Gergely, Pál; Erdodi, Ferenc; Virág, László.

In: Molecular Pharmacology, Vol. 68, No. 3, 09.2005, p. 895-904.

Research output: Contribution to journalArticle

Erdèlyi, K, Kiss, A, Bakondi, E, Bai, P, Szabo, C, Gergely, P, Erdodi, F & Virág, L 2005, 'Gallotannin inhibits the expression of chemokines and inflammatory cytokines in A549 cells', Molecular Pharmacology, vol. 68, no. 3, pp. 895-904. https://doi.org/10.1124/mol.105.012518
Erdèlyi, Katalin ; Kiss, Andrea ; Bakondi, Edina ; Bai, Péter ; Szabo, Csaba ; Gergely, Pál ; Erdodi, Ferenc ; Virág, László. / Gallotannin inhibits the expression of chemokines and inflammatory cytokines in A549 cells. In: Molecular Pharmacology. 2005 ; Vol. 68, No. 3. pp. 895-904.
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AU - Erdèlyi, Katalin

AU - Kiss, Andrea

AU - Bakondi, Edina

AU - Bai, Péter

AU - Szabo, Csaba

AU - Gergely, Pál

AU - Erdodi, Ferenc

AU - Virág, László

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