Gap junctions coordinate the propagation of glycogenolysis induced by norepinephrine in the pineal gland

Eliseo A. Eugenin, Silvana Valdebenito, Anna Maria Gorska, Agustin D. Martínez, Marcela Bitran, Juan C. Sáez

Research output: Contribution to journalArticle

Abstract

Chemical and electrical synapses are the two major communication systems that permit cell-to-cell communication within the nervous system. Although most studies are focused on chemical synapses (glutamate, γ-aminobutyric acid, and other neurotransmitters), clearly both types of synapses interact and cooperate to allow the coordination of several cell functions within the nervous system. The pineal gland has limited independent axonal innervation and not every cell has access to nerve terminals. Thus, additional communication systems, such as gap junctions, have been postulated to coordinate metabolism and signaling. Using acutely isolated glands and dissociated cells, we found that gap junctions spread glycogenolytic signals from cells containing adrenoreceptors to the entire gland lacking these receptors. Our data using glycogen and lactate quantification, electrical stimulation, and high-performance liquid chromatography with electrochemical detection, demonstrate that gap junctional communication between cells of the rat pineal gland allows cell-to-cell propagation of norepinephrine-induced signal that promotes glycogenolysis throughout the entire gland. Thus, the interplay of both synapses is essential for coordinating glycogen metabolism and lactate production in the pineal gland. (Figure presented.).

Original languageEnglish (US)
Pages (from-to)558-569
Number of pages12
JournalJournal of neurochemistry
Volume151
Issue number5
DOIs
StatePublished - Dec 1 2019

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Glycogenolysis
Pineal Gland
Gap Junctions
Neurology
Glycogen
Metabolism
Lactic Acid
Norepinephrine
Communication systems
Aminobutyrates
Communication
High performance liquid chromatography
Neurotransmitter Agents
Rats
Glutamic Acid
Synapses
Cells
Cell Communication
Nervous System
Electrical Synapses

Keywords

  • connexin
  • electrical and chemical synapses
  • glycogen
  • hemichannels

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Gap junctions coordinate the propagation of glycogenolysis induced by norepinephrine in the pineal gland. / Eugenin, Eliseo A.; Valdebenito, Silvana; Gorska, Anna Maria; Martínez, Agustin D.; Bitran, Marcela; Sáez, Juan C.

In: Journal of neurochemistry, Vol. 151, No. 5, 01.12.2019, p. 558-569.

Research output: Contribution to journalArticle

Eugenin, Eliseo A. ; Valdebenito, Silvana ; Gorska, Anna Maria ; Martínez, Agustin D. ; Bitran, Marcela ; Sáez, Juan C. / Gap junctions coordinate the propagation of glycogenolysis induced by norepinephrine in the pineal gland. In: Journal of neurochemistry. 2019 ; Vol. 151, No. 5. pp. 558-569.
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