Gas chromatographic mass spectrometric analysis of biogenic amines in identified neruons and tissues of Hirudo medicinalis

D. J. McAdoo, R. E. Coggeshall

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Abstract

Previous workers have reported that the colossal cells of Retzius in the segmental ganglia of the medicinal leech contain about 2.3 pmol of 5 hydroxytryptamine (5 HT)/cell body. The authors verify the identify of 5 HT in the Retzius cells by gas chromatography mass spectrometry and derive concentrations of 1.3-4.1 pmol/neuron by analyses of eight individually dissected Retzius cell bodies. The Retzius cell bodies contain about 30% of the 5 HT in each ganglion. An average of 25 pmol 5 HT/mg tissue, a concentration about 500 times lower than that in the Retzius cell, was found in the fibrous, pigmented tissue surrounding the leech nervous system. The authors could not detect γ aminobutyric acid, octopamine, dopamine or norepinephrine in the Retzius cells, in the pigmented tissue, or, with the possible exception of dopamine (≤ 0.4 pmol/ganglion), in whole ganglia. Furthermore, they could not detect 5 HT in pooled samples of 100 non chromaffin control neurons.

Original languageEnglish (US)
Pages (from-to)163-167
Number of pages5
JournalJournal of Neurochemistry
Volume26
Issue number1
StatePublished - 1976

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ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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