Previous workers have reported that the colossal cells of Retzius in the segmental ganglia of the medicinal leech contain about 2.3 pmol of 5 hydroxytryptamine (5 HT)/cell body. The authors verify the identify of 5 HT in the Retzius cells by gas chromatography mass spectrometry and derive concentrations of 1.3-4.1 pmol/neuron by analyses of eight individually dissected Retzius cell bodies. The Retzius cell bodies contain about 30% of the 5 HT in each ganglion. An average of 25 pmol 5 HT/mg tissue, a concentration about 500 times lower than that in the Retzius cell, was found in the fibrous, pigmented tissue surrounding the leech nervous system. The authors could not detect γ aminobutyric acid, octopamine, dopamine or norepinephrine in the Retzius cells, in the pigmented tissue, or, with the possible exception of dopamine (≤ 0.4 pmol/ganglion), in whole ganglia. Furthermore, they could not detect 5 HT in pooled samples of 100 non chromaffin control neurons.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of Neurochemistry|
|State||Published - 1976|
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience