Gel electrophoresis, Southern Blot, and colorimetric microwell plate-based system

Jie He, Michael J. Loeffelholz, Jiang Fan

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Infectious disease-related illnesses are a significant threat to human health resulting in substantial morbidity and mortality, worldwide. Timely and accurate diagnostic tools are critical for patient treatment decisions and disease outcomes. Molecular diagnostics are revolutionizing the clinical practice of infectious disease. The various formats of nucleic acid amplification are the most frequently used molecular tests in the diagnosis of infectious diseases due to its exquisite sensitivity and specificity. Gel electrophoresis and Southern hybridization are two basic technologies that are used to display the specific amplification of targeted gene and are still used in the laboratories for diagnosis because it is such a powerful technique, and yet reasonably easy and inexpensive.

Original languageEnglish (US)
Title of host publicationAdvanced Techniques in Diagnostic Microbiology
PublisherSpringer US
Pages367-381
Number of pages15
Volume9781461439707
ISBN (Print)9781461439707, 1461439698, 9781461439691
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

Fingerprint

Southern Blotting
Communicable Diseases
Electrophoresis
Gels
Molecular Pathology
Gene Amplification
Clinical Laboratory Techniques
Nucleic Acids
Technology
Morbidity
Sensitivity and Specificity
Mortality
Health
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology and Microbiology(all)

Cite this

He, J., Loeffelholz, M. J., & Fan, J. (2013). Gel electrophoresis, Southern Blot, and colorimetric microwell plate-based system. In Advanced Techniques in Diagnostic Microbiology (Vol. 9781461439707, pp. 367-381). Springer US. https://doi.org/10.1007/978-1-4614-3970-7_21

Gel electrophoresis, Southern Blot, and colorimetric microwell plate-based system. / He, Jie; Loeffelholz, Michael J.; Fan, Jiang.

Advanced Techniques in Diagnostic Microbiology. Vol. 9781461439707 Springer US, 2013. p. 367-381.

Research output: Chapter in Book/Report/Conference proceedingChapter

He, J, Loeffelholz, MJ & Fan, J 2013, Gel electrophoresis, Southern Blot, and colorimetric microwell plate-based system. in Advanced Techniques in Diagnostic Microbiology. vol. 9781461439707, Springer US, pp. 367-381. https://doi.org/10.1007/978-1-4614-3970-7_21
He J, Loeffelholz MJ, Fan J. Gel electrophoresis, Southern Blot, and colorimetric microwell plate-based system. In Advanced Techniques in Diagnostic Microbiology. Vol. 9781461439707. Springer US. 2013. p. 367-381 https://doi.org/10.1007/978-1-4614-3970-7_21
He, Jie ; Loeffelholz, Michael J. ; Fan, Jiang. / Gel electrophoresis, Southern Blot, and colorimetric microwell plate-based system. Advanced Techniques in Diagnostic Microbiology. Vol. 9781461439707 Springer US, 2013. pp. 367-381
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