Gender differences in contractile and relaxing responses of aortic rings from lipopolysaccharide-treated rats

T. Okawa, Y. P. Vedernokov, G. R. Saade, R. E. Garfield

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Aortic rings from male and female Sprague-Dawley rats were placed in organ chambers filled with Krebs solution, and the isometric tension was recorded. There were no gender differences in responses to potassium chloride or phenylephrine in either intact or endothelium-denuded rings from control animals. Treatment with 10 mg/kg of lipopolysaccharide (LPS) attenuated the contractile response to potassium chloride in intact, but not in endothelium-denuded rings, and suppressed the phenylephrine contractions in rings both with and without endothelium from male and female rats. Endothelium-dependent and -independent relaxation by acetylcholine and the nitric oxide donor diethylamine/NO, respectively, did not depend on gender and were equally suppressed by LPS treatment. Treatment with LPS accentuated the effects of oxyhemoglobin in rings with and without endothelium and this was significantly larger in rings from females compared to males. Thus, gender differences were present in the effect of LPS on vascular responses. LPS induced a greater increase in basal release of nitric oxide and a greater inhibition of depolarization by potassium chloride, but not receptor-activated contractions, in aortic rings from female versus male rats.

Original languageEnglish (US)
Pages (from-to)225-233
Number of pages9
JournalGynecological Endocrinology
Volume16
Issue number3
DOIs
StatePublished - Jan 1 2002

Keywords

  • Aorta
  • Endothelium
  • Gender differences
  • LPS
  • Nitric oxide
  • Oxyhemoglobin

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Obstetrics and Gynecology

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