Gender is a major determinant of the clinical evolution and immune response in hamsters infected with Leishmania spp.

Bruno Travi, Yaneth Osorio, Peter Melby, Bysani Chandrasekar, Lourdes Arteaga, Nancy G. Saravia

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

In regions where leishmaniasis is endemic, clinical disease is usually reported more frequently among males than females. This difference could be due to disparate risks of exposure of males and females, but gender-related differences in the host response to infection may also play a role. Experimental studies of the influence of gender on Leishmania infection have not included parasites of the subgenus Viannia, which is the most common cause of cutaneous leishmaniasis in the Americas. Mice are not readily susceptible to infection by Leishmania (Viannia) spp., but cutaneous infection of hamsters with L. (V.) panamensis or L. (V.) guyanensis resulted in chronic lesions typical of the human disease caused by these parasites. Strikingly, infection of male hamsters resulted in significantly greater lesion size and severity, an increased rate of dissemination to distant cutaneous sites, and a greater parasite burden in the draining lymph node than infection in female animals. Two lines of evidence indicated this gender-related difference in disease evolution was determined at least in part by the sex hormone status of the animal. First, prepubertal male animals had smaller and/or less severe cutaneous lesions than adult male animals. Second, infection of testosterone-treated female animals resulted in significantly larger lesions than in untreated female animals. The increased severity of disease in male compared to female animals was associated with significantly greater intralesional expression of interleukin-4 (IL-4) (P = 0.04), IL-10 (P = 0.04), and transforming growth factor β (TGF-β) (P < 0.001), cytokines known to promote disease in experimental leishmaniasis. There was a direct correlation between the expression of TGF-β mRNA and lesion size (Spearman's correlation coefficient = 0.873; P < 0.001). These findings demonstrate an inherent risk of increased disease severity in male animals, which is associated with a more permissive immune response.

Original languageEnglish (US)
Pages (from-to)2288-2296
Number of pages9
JournalInfection and Immunity
Volume70
Issue number5
DOIs
StatePublished - 2002
Externally publishedYes

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Leishmania
Cricetinae
Infection
Parasites
Leishmaniasis
Transforming Growth Factors
Skin
Endemic Diseases
Cutaneous Leishmaniasis
Gonadal Steroid Hormones
Interleukin-4
Interleukin-10
Testosterone
Lymph Nodes
Cytokines
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

Gender is a major determinant of the clinical evolution and immune response in hamsters infected with Leishmania spp. / Travi, Bruno; Osorio, Yaneth; Melby, Peter; Chandrasekar, Bysani; Arteaga, Lourdes; Saravia, Nancy G.

In: Infection and Immunity, Vol. 70, No. 5, 2002, p. 2288-2296.

Research output: Contribution to journalArticle

Travi, Bruno ; Osorio, Yaneth ; Melby, Peter ; Chandrasekar, Bysani ; Arteaga, Lourdes ; Saravia, Nancy G. / Gender is a major determinant of the clinical evolution and immune response in hamsters infected with Leishmania spp. In: Infection and Immunity. 2002 ; Vol. 70, No. 5. pp. 2288-2296.
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