Gender-related differences in expression of murine glutathione S-transferases and their induction by butylated hydroxyanisole

Meena Chaubey, Sharad S. Singhal, Sanjay Awasthi, Manju Saxena, Roy B. Dyer, Yogesh C. Awasthi, Norbert K. Herzog

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The basal levels of μ and π class glutathione S-transferases RNA were 18-fold higher in the male mouse liver as compared with the female. When 0.75% (w/w) BHA was included in the diet it altered the RNA levels of α, μ, π GST classes and mGSTA4-4 in a tissue and sex specific manner. The most marked induction of RNA was seen for the μ class GSTs of female liver, lung and kidney (52, 10 and 8-fold, respectively), and of male liver and kidney (25 and 3.5-fold, respectively), the π class GSTs of female liver, lung, and kidney (11, 10, and 5-fold, respectively), and mGSTA4-4 of female liver (4-fold). The effect of BHA on the induction of the μ and π class GST RNA was 2-9 fold greater in female as compared with male tissues. The degree of induction of GST RNA did not correlate directly with changes in GST protein indicating that post-transcriptional events regulating GST expression may be affected by BHA particularly for GST μ and mGSTA4-4.

Original languageEnglish (US)
Pages (from-to)311-319
Number of pages9
JournalComparative Biochemistry and Physiology. Part C: Comparative
Volume108
Issue number3
DOIs
StatePublished - 1994

Fingerprint

Butylated Hydroxyanisole
Glutathione Transferase
Liver
RNA
Kidney
Tissue
Lung
Nutrition
Diet
mGSTA4-4
Proteins

Keywords

  • Butylated hydroxyanisole
  • Gene expression
  • Glutathione S-transferase
  • Hybridization
  • Isoforms
  • RNA
  • Sex related expression
  • Xenobiotics

ASJC Scopus subject areas

  • Immunology
  • Pharmacology
  • Toxicology

Cite this

Gender-related differences in expression of murine glutathione S-transferases and their induction by butylated hydroxyanisole. / Chaubey, Meena; Singhal, Sharad S.; Awasthi, Sanjay; Saxena, Manju; Dyer, Roy B.; Awasthi, Yogesh C.; Herzog, Norbert K.

In: Comparative Biochemistry and Physiology. Part C: Comparative, Vol. 108, No. 3, 1994, p. 311-319.

Research output: Contribution to journalArticle

Chaubey, Meena ; Singhal, Sharad S. ; Awasthi, Sanjay ; Saxena, Manju ; Dyer, Roy B. ; Awasthi, Yogesh C. ; Herzog, Norbert K. / Gender-related differences in expression of murine glutathione S-transferases and their induction by butylated hydroxyanisole. In: Comparative Biochemistry and Physiology. Part C: Comparative. 1994 ; Vol. 108, No. 3. pp. 311-319.
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