Gene Expression Patterns in Skeletal Muscle of Thermally Injured Children Treated with Oxandrolone

Robert E. Barrow, Mohan R.K. Dasu, Amy A. Ferrando, Marcus Spies, Steven J. Thomas, J. Regino Perez-Polo, David Herndon

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Objective: To analyze gene expression patterns in skeletal muscle from burned children. Summary Background Data: Analysis of gene expression patterns in skeletal muscle from burned children can help provide a fundamental understanding of muscle wasting at the molecular level. This study is the first to use such an approach in burned children receiving anabolic treatment. Methods: Children who received 0.1 mg/kg oxandrolone twice a day (n = 7) were compared to placebo (n = 7). Net protein balance was determined before and after treatment with oxandrolone. Total RNA, extracted from muscle biopsies obtained from burned children age 3 to 18 years, was purified, reverse transcribed, and biotinylated cRNA hybridized to the human high-density oligonucleotide array (U95Av2). Western blot analysis verified the mRNA changes at their protein level. Results: DNA microarray analysis showed two genes significantly changed in muscle from burned children receiving placebo, while the expression of 21 genes was altered with oxandrolone. Muscle net protein balance increased with oxandrolone treatment compared to placebo. Conclusions: DNA microarray technology will help identify molecular changes that can serve as targets for new therapies to attenuate muscle wasting in severely burned children and thus improve recovery and early rehabilitation.

Original languageEnglish (US)
Pages (from-to)422-428
Number of pages7
JournalAnnals of Surgery
Volume237
Issue number3
DOIs
StatePublished - Jan 1 2003

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Oxandrolone
Skeletal Muscle
Gene Expression
Oligonucleotide Array Sequence Analysis
Muscles
Placebos
Complementary RNA
Muscle Proteins
Gene Expression Profiling
Therapeutics
Microarray Analysis
Proteins
Rehabilitation
Western Blotting
RNA
Technology
Biopsy
Messenger RNA

ASJC Scopus subject areas

  • Surgery

Cite this

Barrow, R. E., Dasu, M. R. K., Ferrando, A. A., Spies, M., Thomas, S. J., Perez-Polo, J. R., & Herndon, D. (2003). Gene Expression Patterns in Skeletal Muscle of Thermally Injured Children Treated with Oxandrolone. Annals of Surgery, 237(3), 422-428. https://doi.org/10.1097/01.SLA.0000055276.10357.FB

Gene Expression Patterns in Skeletal Muscle of Thermally Injured Children Treated with Oxandrolone. / Barrow, Robert E.; Dasu, Mohan R.K.; Ferrando, Amy A.; Spies, Marcus; Thomas, Steven J.; Perez-Polo, J. Regino; Herndon, David.

In: Annals of Surgery, Vol. 237, No. 3, 01.01.2003, p. 422-428.

Research output: Contribution to journalArticle

Barrow, RE, Dasu, MRK, Ferrando, AA, Spies, M, Thomas, SJ, Perez-Polo, JR & Herndon, D 2003, 'Gene Expression Patterns in Skeletal Muscle of Thermally Injured Children Treated with Oxandrolone', Annals of Surgery, vol. 237, no. 3, pp. 422-428. https://doi.org/10.1097/01.SLA.0000055276.10357.FB
Barrow, Robert E. ; Dasu, Mohan R.K. ; Ferrando, Amy A. ; Spies, Marcus ; Thomas, Steven J. ; Perez-Polo, J. Regino ; Herndon, David. / Gene Expression Patterns in Skeletal Muscle of Thermally Injured Children Treated with Oxandrolone. In: Annals of Surgery. 2003 ; Vol. 237, No. 3. pp. 422-428.
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