Gene expression profile of human lymphoid CEM cells sensitive and resistant to glucocorticoid-evoked apoptosis

Rheem D. Medh, M. Scott Webb, Aaron L. Miller, Betty H. Johnson, Yuriy Fofanov, Tongbin Li, Thomas Wood, Bruce A. Luxon, E. Brad Thompson

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Three closely related clones of leukemic lymphoid CEM cells were compared for their gene expression responses to the glucocorticoid dexamethasone (Dex). All three contained receptors for Dex, but only two responded by undergoing apoptosis. After a time of exposure to Dex that ended late in the interval preceding onset of apoptosis, gene microarray analyses were carried out. The results indicate that the expression of a limited, distinctive set of genes was altered in the two apoptosis-prone clones, not in the resistant clone. That clone showed altered expression of different sets of genes, suggesting that a molecular switch converted patterns of gene expression between the two phenotypes: apoptosis-prone and apoptosis-resistant. The results are consistent with the hypothesis that altered expression of a distinctive network of genes after glucocorticoid administration ultimately triggers apoptosis of leukemic lymphoid cells. The altered genes identified provide new foci for study of their role in cell death.

Original languageEnglish (US)
Pages (from-to)543-555
Number of pages13
JournalGenomics
Volume81
Issue number6
DOIs
StatePublished - Jun 1 2003

Fingerprint

Transcriptome
Glucocorticoids
Lymphocytes
Apoptosis
Clone Cells
Dexamethasone
Genes
Gene Expression
Gene Regulatory Networks
Microarray Analysis
Cell Death
Phenotype

Keywords

  • Apoptosis
  • CEM
  • Data mining
  • Gene expression profile
  • Glucocorticoid sensitivity/resistance
  • Human lymphoid cells
  • Microarray analysis
  • Novel genes

ASJC Scopus subject areas

  • Genetics

Cite this

Gene expression profile of human lymphoid CEM cells sensitive and resistant to glucocorticoid-evoked apoptosis. / Medh, Rheem D.; Webb, M. Scott; Miller, Aaron L.; Johnson, Betty H.; Fofanov, Yuriy; Li, Tongbin; Wood, Thomas; Luxon, Bruce A.; Thompson, E. Brad.

In: Genomics, Vol. 81, No. 6, 01.06.2003, p. 543-555.

Research output: Contribution to journalArticle

Medh, RD, Webb, MS, Miller, AL, Johnson, BH, Fofanov, Y, Li, T, Wood, T, Luxon, BA & Thompson, EB 2003, 'Gene expression profile of human lymphoid CEM cells sensitive and resistant to glucocorticoid-evoked apoptosis', Genomics, vol. 81, no. 6, pp. 543-555. https://doi.org/10.1016/S0888-7543(03)00045-4
Medh, Rheem D. ; Webb, M. Scott ; Miller, Aaron L. ; Johnson, Betty H. ; Fofanov, Yuriy ; Li, Tongbin ; Wood, Thomas ; Luxon, Bruce A. ; Thompson, E. Brad. / Gene expression profile of human lymphoid CEM cells sensitive and resistant to glucocorticoid-evoked apoptosis. In: Genomics. 2003 ; Vol. 81, No. 6. pp. 543-555.
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