Gene Sequence-Based Criteria for Identification of New Rickettsia Isolates and Description of Rickettsia heilongjiangensis sp. nov.

Pierre Edouard Fournier, J. Stephen Dumler, Gilbert Greub, Jianzhi Zhang, Yimin Wu, Didier Raoult

    Research output: Contribution to journalArticle

    244 Scopus citations

    Abstract

    We propose genetic guidelines for the classification of rickettsial isolates at the genus, group, and species levels by using sequences of the 16S rRNA (rrs) gene and four protein-coding genes, the gltA, ompA, and ompB genes and gene D. To be classified as a member of the genus Rickettsia, an isolate should exhibit degrees of rrs and gita homology with any of the 20 Rickettsia species studied of ≥98.1 and ≥86.5%, respectively. A member of the typhus group should fulfill at least two of the following four criteria: Pairwise nucleotide sequence homologies with rrs, gltA, ompB, and gene D of either Rickettsia typhi or Rickettsia prowazekii of ≥99.4, ≥96.6, ≥92.4, and ≥91.6%, respectively. A member of the spotted fever group should either possess the ompA gene of or fulfill at least two of the following four criteria: pairwise nucleotide sequence homologies with rrs, gltA, ompB, and gene D of any member of this group of ≥98.8, ≥92.7, ≥85.8, and ≥82.2%, respectively. The existence of a distinct "ancestral" group should be questioned. To be classified as a new Rickettsia species, an isolate should not exhibit more than one of the following degrees of nucleotide similarity with the most homologous validated species: ≥99.8 and ≥99.9% for the rrs and gltA genes, respectively, and, when amplifiable, ≥98.8, ≥99.2% and ≥99.3% for the ompA and ompB genes and gene D, respectively. By use of our classification scheme, "Rickettsia heilongjiangii" belongs to a new species for which we officially propose the name Rickettsia heilongjiangensis sp. nov.

    Original languageEnglish (US)
    Pages (from-to)5456-5465
    Number of pages10
    JournalJournal of Clinical Microbiology
    Volume41
    Issue number12
    DOIs
    StatePublished - Dec 1 2003

    ASJC Scopus subject areas

    • Microbiology (medical)

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