Gene Sequence-Based Criteria for Identification of New Rickettsia Isolates and Description of Rickettsia heilongjiangensis sp. nov.

Pierre Edouard Fournier, J. Stephen Dumler, Gilbert Greub, Jianzhi Zhang, Yimin Wu, Didier Raoult

Research output: Contribution to journalArticle

230 Citations (Scopus)

Abstract

We propose genetic guidelines for the classification of rickettsial isolates at the genus, group, and species levels by using sequences of the 16S rRNA (rrs) gene and four protein-coding genes, the gltA, ompA, and ompB genes and gene D. To be classified as a member of the genus Rickettsia, an isolate should exhibit degrees of rrs and gita homology with any of the 20 Rickettsia species studied of ≥98.1 and ≥86.5%, respectively. A member of the typhus group should fulfill at least two of the following four criteria: Pairwise nucleotide sequence homologies with rrs, gltA, ompB, and gene D of either Rickettsia typhi or Rickettsia prowazekii of ≥99.4, ≥96.6, ≥92.4, and ≥91.6%, respectively. A member of the spotted fever group should either possess the ompA gene of or fulfill at least two of the following four criteria: pairwise nucleotide sequence homologies with rrs, gltA, ompB, and gene D of any member of this group of ≥98.8, ≥92.7, ≥85.8, and ≥82.2%, respectively. The existence of a distinct "ancestral" group should be questioned. To be classified as a new Rickettsia species, an isolate should not exhibit more than one of the following degrees of nucleotide similarity with the most homologous validated species: ≥99.8 and ≥99.9% for the rrs and gltA genes, respectively, and, when amplifiable, ≥98.8, ≥99.2% and ≥99.3% for the ompA and ompB genes and gene D, respectively. By use of our classification scheme, "Rickettsia heilongjiangii" belongs to a new species for which we officially propose the name Rickettsia heilongjiangensis sp. nov.

Original languageEnglish (US)
Pages (from-to)5456-5465
Number of pages10
JournalJournal of Clinical Microbiology
Volume41
Issue number12
DOIs
StatePublished - Dec 2003

Fingerprint

Rickettsia
Genes
Sequence Homology
Rickettsia typhi
Rickettsia prowazekii
Epidemic Louse-Borne Typhus
rRNA Genes
Names
Fever
Nucleotides
Guidelines

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Gene Sequence-Based Criteria for Identification of New Rickettsia Isolates and Description of Rickettsia heilongjiangensis sp. nov. / Fournier, Pierre Edouard; Dumler, J. Stephen; Greub, Gilbert; Zhang, Jianzhi; Wu, Yimin; Raoult, Didier.

In: Journal of Clinical Microbiology, Vol. 41, No. 12, 12.2003, p. 5456-5465.

Research output: Contribution to journalArticle

Fournier, Pierre Edouard ; Dumler, J. Stephen ; Greub, Gilbert ; Zhang, Jianzhi ; Wu, Yimin ; Raoult, Didier. / Gene Sequence-Based Criteria for Identification of New Rickettsia Isolates and Description of Rickettsia heilongjiangensis sp. nov. In: Journal of Clinical Microbiology. 2003 ; Vol. 41, No. 12. pp. 5456-5465.
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