GeneChip analyses of global transcriptional responses of murine macrophages to the lethal toxin of Bacillus anthracis

Jason E. Comer; Cristi L. Galindo; Ashok K. Chopra; Johnny Peterson

doi:10.1128/IAI.73.3.1879-1885.2005

GeneChip analyses of global transcriptional responses of murine macrophages to the lethal toxin of Bacillus anthracis

Jason E. Comer, Cristi L. Galindo, Ashok K. Chopra, Johnny Peterson

Microbiology And Immunology

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

We performed GeneChip analyses on RNA from Bacillus anthracis lethal toxin (LeTx)-treated RAW 264.7 murine macrophages to investigate global effects of anthrax toxin on host cell gene expression. Stringent analysis of data revealed that the expression of several mitogen-activated protein kinase kinase-regulatory genes was affected within 1.5 h post-exposure to LeTx. By 3.0 h, the expression of 103 genes was altered, including those involved in intracellular signaling, energy production, and protein metabolism.

Original language	English (US)
Pages (from-to)	1879-1885
Number of pages	7
Journal	Infection and immunity
Volume	73
Issue number	3
DOIs	https://doi.org/10.1128/IAI.73.3.1879-1885.2005
State	Published - Mar 2005

ASJC Scopus subject areas

Parasitology
Microbiology
Immunology
Infectious Diseases

Access to Document

10.1128/IAI.73.3.1879-1885.2005

Cite this

@article{bbc139c663d048e0a44b8d27da72c6a7,

title = "GeneChip analyses of global transcriptional responses of murine macrophages to the lethal toxin of Bacillus anthracis",

abstract = "We performed GeneChip analyses on RNA from Bacillus anthracis lethal toxin (LeTx)-treated RAW 264.7 murine macrophages to investigate global effects of anthrax toxin on host cell gene expression. Stringent analysis of data revealed that the expression of several mitogen-activated protein kinase kinase-regulatory genes was affected within 1.5 h post-exposure to LeTx. By 3.0 h, the expression of 103 genes was altered, including those involved in intracellular signaling, energy production, and protein metabolism.",

author = "Comer, {Jason E.} and Galindo, {Cristi L.} and Chopra, {Ashok K.} and Johnny Peterson",

year = "2005",

month = mar,

doi = "10.1128/IAI.73.3.1879-1885.2005",

language = "English (US)",

volume = "73",

pages = "1879--1885",

journal = "Infection and immunity",

issn = "0019-9567",

publisher = "American Society for Microbiology",

number = "3",

}

TY - JOUR

T1 - GeneChip analyses of global transcriptional responses of murine macrophages to the lethal toxin of Bacillus anthracis

AU - Comer, Jason E.

AU - Galindo, Cristi L.

AU - Chopra, Ashok K.

AU - Peterson, Johnny

PY - 2005/3

Y1 - 2005/3

N2 - We performed GeneChip analyses on RNA from Bacillus anthracis lethal toxin (LeTx)-treated RAW 264.7 murine macrophages to investigate global effects of anthrax toxin on host cell gene expression. Stringent analysis of data revealed that the expression of several mitogen-activated protein kinase kinase-regulatory genes was affected within 1.5 h post-exposure to LeTx. By 3.0 h, the expression of 103 genes was altered, including those involved in intracellular signaling, energy production, and protein metabolism.

AB - We performed GeneChip analyses on RNA from Bacillus anthracis lethal toxin (LeTx)-treated RAW 264.7 murine macrophages to investigate global effects of anthrax toxin on host cell gene expression. Stringent analysis of data revealed that the expression of several mitogen-activated protein kinase kinase-regulatory genes was affected within 1.5 h post-exposure to LeTx. By 3.0 h, the expression of 103 genes was altered, including those involved in intracellular signaling, energy production, and protein metabolism.

UR - http://www.scopus.com/inward/record.url?scp=15544367374&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=15544367374&partnerID=8YFLogxK

U2 - 10.1128/IAI.73.3.1879-1885.2005

DO - 10.1128/IAI.73.3.1879-1885.2005

M3 - Article

C2 - 15731093

AN - SCOPUS:15544367374

SN - 0019-9567

VL - 73

SP - 1879

EP - 1885

JO - Infection and immunity

JF - Infection and immunity

IS - 3

ER -

GeneChip analyses of global transcriptional responses of murine macrophages to the lethal toxin of Bacillus anthracis

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this