Generation and characterization of a novel transgenic mouse harboring conditional nuclear factor-kappa B/RelA knockout alleles

Talha Ijaz, Maki Wakamiya, Hong Sun, Adrian Recinos, Ronald Tilton, Allan R. Brasier

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Nuclear Factor-Kappa B (NF-kB) is a family of transcription factors that are important in embryonic development, inflammation, epithelial-to-mesenchymal transition and cancer. The 65 kDa RelA subunit is the major transcriptional activator of the NF-kB pathways. Whole-body deficiency of RelA leads to massive apoptosis of liver hepatocytes and death in utero. To study the role of RelA in physiology and in disease states in a manner that circumvents this embryonic lethal phenotype, we have generated a mouse with RelA conditional knockout (CKO) alleles containing loxP sites that are deleted by activated Cre recombinase. Results: We demonstrate that RelACKO/CKO mice are fertile, do not display any developmental defects and can be crossed with Cre-expressing mice to delete RelA in a temporal, tissue-specific manner. Our mating of RelACKO/CKO mice with Zp3-Cre transgenic led to embryonic lethality of RelA-deficient embryos. In contrast, mating of RelACKO/CKO mice with Col1α2-CreER mice allowed for the generation of double transgenics which could be stimulated with tamoxifen to induce fibroblast-specific RelA deletion in adulthood. Conclusions: Based on our collective data, we conclude that this novel RelACKO/CKO mouse allows for efficient deletion of RelA in a tissue-specific manner. This RelACKO/CKO mouse will be an invaluable tool for deciphering the mechanistic roles of RelA in various cells and tissues during development and in disease.

Original languageEnglish (US)
Article number32
JournalBMC Developmental Biology
Volume16
Issue number1
DOIs
StatePublished - Sep 23 2016

Fingerprint

NF-kappa B
Knockout Mice
Transgenic Mice
Alleles
Epithelial-Mesenchymal Transition
Tamoxifen
Embryonic Development
Hepatocytes
Transcription Factors
Embryonic Structures
Fibroblasts
Apoptosis
Inflammation
Phenotype
Liver
Neoplasms

Keywords

  • Col1a2
  • Cre
  • Flox
  • NF-kB
  • p65
  • RelA
  • Tamoxifen

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Generation and characterization of a novel transgenic mouse harboring conditional nuclear factor-kappa B/RelA knockout alleles. / Ijaz, Talha; Wakamiya, Maki; Sun, Hong; Recinos, Adrian; Tilton, Ronald; Brasier, Allan R.

In: BMC Developmental Biology, Vol. 16, No. 1, 32, 23.09.2016.

Research output: Contribution to journalArticle

Ijaz, Talha ; Wakamiya, Maki ; Sun, Hong ; Recinos, Adrian ; Tilton, Ronald ; Brasier, Allan R. / Generation and characterization of a novel transgenic mouse harboring conditional nuclear factor-kappa B/RelA knockout alleles. In: BMC Developmental Biology. 2016 ; Vol. 16, No. 1.
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