Generation and characterization of West Nile pseudo-infectious reporter virus for antiviral screening

Hong Lei Zhang, Han Qing Ye, Cheng Lin Deng, Si Qing Liu, Pei Yong Shi, Cheng Feng Qin, Zhi Ming Yuan, Bo Zhang

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


West Nile virus (WNV), a mosquito-borne flavivirus, is an important neurotropic human pathogen. As a biosafety level-3 (BSL-3) agent, WNV is strictly to BSL-3 laboratories for experimentations, thus greatly hindering the development of vaccine and antiviral drug. Here, we developed a novel pseudo-infectious WNV reporter virus expressing the Gaussia luciferase (Gluc). A stable 293TNS1 cell line expressing NS1 was selected for trans-supplying NS1 protein to support the replication of WNV-ΔNS1 virus and WNV-ΔNS1-Gluc reporter virus with large-fragment deletion of NS1. WNV-ΔNS1 virus and WNV-Gluc-ΔNS1 reporter virus were confined to complete their replication cycle in this 293TNS1 cell line, displaying nearly identical growth kinetics to WT WNV although the viral titers were lower than those of WT WNV. The reporter gene was stably maintained in virus genome at least within three rounds of passage in 293TNS1 cell line. Using a known flaviviruses inhibitor, NITD008, we demonstrated that the pseudo-infectious WNV-Gluc-ΔNS1 could be used for antiviral screening. Furthermore, a high-throughput screening (HTS) assay in a 96-well format was optimized and validated using several known WNV inhibitors, indicating that the optimized HTS assay was suitable for high-throughput screening WNV inhibitors. Our work provides a stable and safe tool to handle WNV outside of a BSL-3 facility and facilitates high throughput screening for anti-WNV drugs.

Original languageEnglish (US)
Pages (from-to)38-47
Number of pages10
JournalAntiviral research
StatePublished - May 1 2017


  • Antiviral screening
  • BSL-3
  • Pseudo-infectious virus
  • Reporter virus
  • West Nile virus

ASJC Scopus subject areas

  • Pharmacology
  • Virology


Dive into the research topics of 'Generation and characterization of West Nile pseudo-infectious reporter virus for antiviral screening'. Together they form a unique fingerprint.

Cite this