Generation and detection of hydroxyl radical in vivo in rat spinal cord by microdialysis administration sampling

Danxia Liu

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We developed a double microdialysis fiber technique to generate hydroxyl radicals (OH) in rat spinal cord. H2O2 and FeCl2/EDTA were pumped through two parallel microdialysis fibers inserted into the spinal cord such that the reactants mix in the tissue to generate OH by the Fenton reaction. Generated OH was detected by administering phenylalanine through one fiber and measuring o-,m- and p-hydroxyphenylalanine in collected, dialysates by high pressure liquid chromatography and fluorescence detection. The hydroxyphenylalanines are produced by OH attacking the phenylalanine. OH generation was also accomplished in in vitro experiments and the results were consistent with in vivo experiments. This novel method to generate and measure OH radical in vivo overcomes difficulties in studying damage to tissue by short-lived OH. Although developed to study the role of OH in spinal cord injury, this method could be used to study other diseases involving OH damage.

Original languageEnglish (US)
Pages (from-to)281-291
Number of pages11
JournalJournal of Biochemical and Biophysical Methods
Volume27
Issue number4
DOIs
StatePublished - 1993

Fingerprint

Microdialysis
Phenylalanine
Hydroxyl Radical
Rats
Spinal Cord
Sampling
Fibers
Dialysis Solutions
Spinal Cord Injuries
Edetic Acid
High pressure liquid chromatography
Tissue
Fluorescence
High Pressure Liquid Chromatography
Experiments
In Vitro Techniques

Keywords

  • Fluorescence detection
  • High pressure liquid chromatography
  • Hydroxyl radical generation
  • Microdialysisl Fenton reaction
  • Phenylalanine hydroxylation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Generation and detection of hydroxyl radical in vivo in rat spinal cord by microdialysis administration sampling. / Liu, Danxia.

In: Journal of Biochemical and Biophysical Methods, Vol. 27, No. 4, 1993, p. 281-291.

Research output: Contribution to journalArticle

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