Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre

Teresa Gallardo, Lane Shirley, George B. John, Diego H. Castrillon

Research output: Contribution to journalArticle

183 Citations (Scopus)

Abstract

Cell type-specific genetic modification using the Cre/loxP system is a powerful tool for genetic analysis of distinct cell lineages. Because of the exquisite specificity of Vasa expression (confined to the germ cell lineage in invertebrate and vertebrate species), we hypothesized that a Vasa promoter-driven transgenic Cre line would prove useful for the germ cell lineage-specific inactivation of genes. Here we describe a transgenic mouse line, Vasa-Cre, where Cre is efficiently and specifically expressed in germ cells. Northern analysis showed that transgene expression was confined to the gonads. Cre-mediated recombination with the Rosa26-lacZ reporter was observed beginning at ∼e15, and was >95% efficient in male and female germ cells by birth. Although there was a potent maternal effect with some animals showing more widespread recombination, there was no ectopic activity in most adults. This Vasa-Cre transgenic line should thus prove useful for genetic analysis of diverse aspects of gametogenesis and as a general deletor line.

Original languageEnglish (US)
Pages (from-to)413-417
Number of pages5
JournalGenesis
Volume45
Issue number6
DOIs
StatePublished - Jun 2007
Externally publishedYes

Fingerprint

Germ Cells
Transgenic Mice
Cell Lineage
Genetic Recombination
Gametogenesis
Gonads
Gene Silencing
Invertebrates
Transgenes
Vertebrates
Parturition

Keywords

  • Cre
  • Germ cells
  • Gonad
  • Vasa

ASJC Scopus subject areas

  • Genetics

Cite this

Gallardo, T., Shirley, L., John, G. B., & Castrillon, D. H. (2007). Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre. Genesis, 45(6), 413-417. https://doi.org/10.1002/dvg.20310

Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre. / Gallardo, Teresa; Shirley, Lane; John, George B.; Castrillon, Diego H.

In: Genesis, Vol. 45, No. 6, 06.2007, p. 413-417.

Research output: Contribution to journalArticle

Gallardo, T, Shirley, L, John, GB & Castrillon, DH 2007, 'Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre', Genesis, vol. 45, no. 6, pp. 413-417. https://doi.org/10.1002/dvg.20310
Gallardo T, Shirley L, John GB, Castrillon DH. Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre. Genesis. 2007 Jun;45(6):413-417. https://doi.org/10.1002/dvg.20310
Gallardo, Teresa ; Shirley, Lane ; John, George B. ; Castrillon, Diego H. / Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre. In: Genesis. 2007 ; Vol. 45, No. 6. pp. 413-417.
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