Generation of an infectious Negev virus cDNA clone

Rodion V. Gorchakov, Robert B. Tesh, Scott C. Weaver, Farooq Nasar

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The genus Negevirus consists of insect-only viruses isolated from mosquitoes and sandflies. Here, we report the successful construction of a full-length infectious cDNA clone of Negev virus (NEGV) strain M30957. Viral RNA was transcribed in vitro and virus was readily rescued with or without the use of a cap analogue. These results strongly suggest that NEGV, and likely other members within the genus, is a non-segmented, single-stranded, positive-sense RNA virus.

Original languageEnglish (US)
Pages (from-to)2071-2074
Number of pages4
JournalJournal of General Virology
Volume95
Issue numberPART 9
DOIs
StatePublished - Sep 2014

ASJC Scopus subject areas

  • Virology

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