Generation of an infectious Negev virus cDNA clone

Rodion V. Gorchakov, Robert B. Tesh, Scott Weaver, Farooq Nasar

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The genus Negevirus consists of insect-only viruses isolated from mosquitoes and sandflies. Here, we report the successful construction of a full-length infectious cDNA clone of Negev virus (NEGV) strain M30957. Viral RNA was transcribed in vitro and virus was readily rescued with or without the use of a cap analogue. These results strongly suggest that NEGV, and likely other members within the genus, is a non-segmented, single-stranded, positive-sense RNA virus.

Original languageEnglish (US)
Pages (from-to)2071-2074
Number of pages4
JournalJournal of General Virology
Volume95
Issue numberPART 9
DOIs
StatePublished - 2014

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Complementary DNA
Clone Cells
Viruses
Insect Viruses
Psychodidae
RNA Viruses
Viral RNA
Culicidae
In Vitro Techniques

ASJC Scopus subject areas

  • Virology

Cite this

Generation of an infectious Negev virus cDNA clone. / Gorchakov, Rodion V.; Tesh, Robert B.; Weaver, Scott; Nasar, Farooq.

In: Journal of General Virology, Vol. 95, No. PART 9, 2014, p. 2071-2074.

Research output: Contribution to journalArticle

Gorchakov, Rodion V. ; Tesh, Robert B. ; Weaver, Scott ; Nasar, Farooq. / Generation of an infectious Negev virus cDNA clone. In: Journal of General Virology. 2014 ; Vol. 95, No. PART 9. pp. 2071-2074.
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