TY - JOUR
T1 - Generation of an infectious Negev virus cDNA clone
AU - Gorchakov, Rodion V.
AU - Tesh, Robert B.
AU - Weaver, Scott C.
AU - Nasar, Farooq
PY - 2014/9
Y1 - 2014/9
N2 - The genus Negevirus consists of insect-only viruses isolated from mosquitoes and sandflies. Here, we report the successful construction of a full-length infectious cDNA clone of Negev virus (NEGV) strain M30957. Viral RNA was transcribed in vitro and virus was readily rescued with or without the use of a cap analogue. These results strongly suggest that NEGV, and likely other members within the genus, is a non-segmented, single-stranded, positive-sense RNA virus.
AB - The genus Negevirus consists of insect-only viruses isolated from mosquitoes and sandflies. Here, we report the successful construction of a full-length infectious cDNA clone of Negev virus (NEGV) strain M30957. Viral RNA was transcribed in vitro and virus was readily rescued with or without the use of a cap analogue. These results strongly suggest that NEGV, and likely other members within the genus, is a non-segmented, single-stranded, positive-sense RNA virus.
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U2 - 10.1099/vir.0.066019-0
DO - 10.1099/vir.0.066019-0
M3 - Article
C2 - 24878640
AN - SCOPUS:84906216189
SN - 0022-1317
VL - 95
SP - 2071
EP - 2074
JO - Journal of General Virology
JF - Journal of General Virology
IS - PART 9
ER -