@article{b8610c06606d4196a1599c25c6665289,
title = "Generation of bat-derived influenza viruses and their reassortants",
abstract = "Two novel influenza A virus-like genomes were detected in fruit bats in Central and South America. However, the biological properties of these bat-derived influenza viruses (BatIVs) are still largely unknown since infectious viral particles have never been isolated from the infected host species. In this study, a reverse genetics approach was used to generate infectious BatIV particles entirely from plasmids encoding full-length sequences in eight gene segments. We inoculated BatIV particles into various cell cultures including bat-derived cell lines and found that BatIVs infected particular bat-derived cells efficiently but not the other cell lines tested. Reassortant viruses between the two BatIVs were also successfully generated and their replication in the susceptible bat cell lines was confirmed. These findings suggest a limited host range and reassortment potential of BatIVs in nature, providing fundamental information for understanding of the ecology of BatIVs.",
author = "Masahiro Sato and Junki Maruyama and Tatsunari Kondoh and Naganori Nao and Hiroko Miyamoto and Yoshihiro Takadate and Wakako Furuyama and Masahiro Kajihara and Hirohito Ogawa and Rashid Manzoor and Reiko Yoshida and Manabu Igarashi and Ayato Takada",
note = "Funding Information: We thank A. Shigeno for technical assistance, Suxiang Tong, Yan Li and Ken Maeda for material information, and K. Barrymore for editing the manuscript. This work was supported by KAKENHI (16H02627 and 15H01249), a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), and partly by the Japan Initiative for Global Research Network on Infectious Diseases (J-GRID) and the Japan Agency for Medical Research and Development (AMED) and Japan International Cooperation Agency (JICA) under the framework of the Science and Technology Research Partnership for Sustainable Development (SATREPS). Publisher Copyright: {\textcopyright} 2019, The Author(s).",
year = "2019",
month = dec,
day = "1",
doi = "10.1038/s41598-018-37830-x",
language = "English (US)",
volume = "9",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}