Generation of bat-derived influenza viruses and their reassortants

Masahiro Sato, Junki Maruyama, Tatsunari Kondoh, Naganori Nao, Hiroko Miyamoto, Yoshihiro Takadate, Wakako Furuyama, Masahiro Kajihara, Hirohito Ogawa, Rashid Manzoor, Reiko Yoshida, Manabu Igarashi, Ayato Takada

Research output: Contribution to journalArticle

Abstract

Two novel influenza A virus-like genomes were detected in fruit bats in Central and South America. However, the biological properties of these bat-derived influenza viruses (BatIVs) are still largely unknown since infectious viral particles have never been isolated from the infected host species. In this study, a reverse genetics approach was used to generate infectious BatIV particles entirely from plasmids encoding full-length sequences in eight gene segments. We inoculated BatIV particles into various cell cultures including bat-derived cell lines and found that BatIVs infected particular bat-derived cells efficiently but not the other cell lines tested. Reassortant viruses between the two BatIVs were also successfully generated and their replication in the susceptible bat cell lines was confirmed. These findings suggest a limited host range and reassortment potential of BatIVs in nature, providing fundamental information for understanding of the ecology of BatIVs.

Original languageEnglish (US)
Article number1158
JournalScientific Reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

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Orthomyxoviridae
Cell Line
Reassortant Viruses
Reverse Genetics
Central America
South America
Host Specificity
Influenza A virus
Ecology
Virion
Fruit
Plasmids
Cell Culture Techniques
Genome
Genes

ASJC Scopus subject areas

  • General

Cite this

Sato, M., Maruyama, J., Kondoh, T., Nao, N., Miyamoto, H., Takadate, Y., ... Takada, A. (2019). Generation of bat-derived influenza viruses and their reassortants. Scientific Reports, 9(1), [1158]. https://doi.org/10.1038/s41598-018-37830-x

Generation of bat-derived influenza viruses and their reassortants. / Sato, Masahiro; Maruyama, Junki; Kondoh, Tatsunari; Nao, Naganori; Miyamoto, Hiroko; Takadate, Yoshihiro; Furuyama, Wakako; Kajihara, Masahiro; Ogawa, Hirohito; Manzoor, Rashid; Yoshida, Reiko; Igarashi, Manabu; Takada, Ayato.

In: Scientific Reports, Vol. 9, No. 1, 1158, 01.12.2019.

Research output: Contribution to journalArticle

Sato, M, Maruyama, J, Kondoh, T, Nao, N, Miyamoto, H, Takadate, Y, Furuyama, W, Kajihara, M, Ogawa, H, Manzoor, R, Yoshida, R, Igarashi, M & Takada, A 2019, 'Generation of bat-derived influenza viruses and their reassortants', Scientific Reports, vol. 9, no. 1, 1158. https://doi.org/10.1038/s41598-018-37830-x
Sato, Masahiro ; Maruyama, Junki ; Kondoh, Tatsunari ; Nao, Naganori ; Miyamoto, Hiroko ; Takadate, Yoshihiro ; Furuyama, Wakako ; Kajihara, Masahiro ; Ogawa, Hirohito ; Manzoor, Rashid ; Yoshida, Reiko ; Igarashi, Manabu ; Takada, Ayato. / Generation of bat-derived influenza viruses and their reassortants. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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