Generation of monoclonal antibody against protein phosphatase 5

O. M. Malanchuk, G. V. Ovcharenko, I. O. Tykhonkova, O. S. Khoma, U. A. Chorna, M. Yu Rybak, S. S. Palchevskyy

Research output: Contribution to journalArticlepeer-review


Aim. Serine/threonine protein phosphatase 5 (PP5) is a unique member of serine/threonine phosphatase family, comprises a regulatory tetratricopeptide repeat (TPR) domain and regulates many cell signaling pathways. Here, we describe the development of a PP5 specific monoclonal antibody (mAb) and characterize its suitability for Western blotting and immunoprecipitation. Methods. Hybridoma technology has been used for monoclonal antibody generation. Immunization was carried out with recombinant mouse PP5 expressed in Escherichia coli as a GST-tagged fusion protein. Results. mAb against PP5 has been generated. Conclusions. Generated mAb specifically recognizes recombinant and endogenous mouse and rat PP5 and is suitable for Western blotting and immunoprecipitation. This mAb will be useful tool for investigations of PP5 physiological role.

Original languageEnglish (US)
Pages (from-to)136-142
Number of pages7
JournalBiopolymers and Cell
Issue number2
StatePublished - 2013
Externally publishedYes


  • Hybridoma technique
  • Monoclonal antibody
  • Protein phosphatase 5

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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