Generation of reactive oxygen and nitrogen species in contracting skeletal muscle

Potential impact on aging

Michael B. Reid, William J. Durham

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Since the early 1980s biologists have recognized that skeletal muscle generates free radicals. Of particular interest are two closely related redox cascades-reactive oxygen species (RES) and nitric oxide (NO) derivatives. The Res cascade is initiated by superoxide anion radicals derived from the mitochondrial electron transport chain, the membrane-associated NAD(P)H oxidase complex, or other sources. NO is produced by two NO synthase isoforms constitutively expressed by muscle fibers. Res and NO derivatives are produced continually and are detectable in both the cytosolic and extracellular compartments. Production increases during strenuous exercise. Both Res and NO modulate contractile function. Under basal conditions, low levels of ROS enhance force production. Excessive Res accumulation inhibits force, for example, during fatiguing exercise. NO inhibits skeletal muscle contraction, an effect that is partially mediated by cyclic GMP as a second messenger. With aging, redox modulation of muscle contraction may be altered by changes in the rates of ROS and NO production, the levels of endogenous antioxidants that buffer ROS and NO, and the sensitivities of regulatory proteins to ROS and NO action. The impact of aging on contractile regulation depends on the relative magnitude of these changes and their net effects on ROS and NO activities at the cellular level.

Original languageEnglish (US)
Pages (from-to)108-116
Number of pages9
JournalAnnals of the New York Academy of Sciences
Volume959
StatePublished - 2002
Externally publishedYes

Fingerprint

Reactive Nitrogen Species
Muscle
Reactive Oxygen Species
Nitric Oxide
Skeletal Muscle
Aging of materials
Muscle Contraction
Superoxides
Oxidation-Reduction
Derivatives
Exercise
Contraction
Nitrogen
Oxygen
NADPH Oxidase
Cyclic GMP
Second Messenger Systems
Electron Transport
Nitric Oxide Synthase
Free Radicals

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Generation of reactive oxygen and nitrogen species in contracting skeletal muscle : Potential impact on aging. / Reid, Michael B.; Durham, William J.

In: Annals of the New York Academy of Sciences, Vol. 959, 2002, p. 108-116.

Research output: Contribution to journalArticle

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