Generation serological assay for monitoring HIV exposure

Miles W. Cloyd, Keith M. Ramsey

Research output: Contribution to journalArticle

Abstract

Disclosed is a highly sensitive anti-HIV antibody detection assay. The assay detects the presence of anti-HIV antibodies through the use of a non-denatured HIV antigenic determinant which immunoreactivity binds anti-HIV antibodies in a biological sample. The non-denatured HIV antigenic determinant has provided a means for detecting anti-HIV antibodies in serum samples testing seronegative for the presence of HIV antibodies directed against denatured HIV antigens p17, p19, p24, p27, p39, gp41, p55, gp120 and gp160. The antigenic determinant may take the form of a cross-immunoreactive live HIV-infected cell line or a biologically engineered peptide possessing conserved or shared HIV surface antigenic determinants. Methods of preparing HIV target antigens are also disclosed, as well as methods for determining the presence of anti-HIV antibodies through the use of these non-denatured HIV target antigens. A test kit for detecting anti-HIV antibodies is also disclosed.

Original languageEnglish (US)
Pages (from-to)703
Number of pages1
JournalBiotechnology Advances
Volume15
Issue number3-4
StatePublished - 1997

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HIV Antibodies
Antibodies
Assays
Anti-Idiotypic Antibodies
HIV
HIV Antigens
Monitoring
Epitopes
Antigens
Peptides
Cells
Cell Line
Testing
Serum

ASJC Scopus subject areas

  • Biotechnology

Cite this

Cloyd, M. W., & Ramsey, K. M. (1997). Generation serological assay for monitoring HIV exposure. Biotechnology Advances, 15(3-4), 703.

Generation serological assay for monitoring HIV exposure. / Cloyd, Miles W.; Ramsey, Keith M.

In: Biotechnology Advances, Vol. 15, No. 3-4, 1997, p. 703.

Research output: Contribution to journalArticle

Cloyd, MW & Ramsey, KM 1997, 'Generation serological assay for monitoring HIV exposure', Biotechnology Advances, vol. 15, no. 3-4, pp. 703.
Cloyd, Miles W. ; Ramsey, Keith M. / Generation serological assay for monitoring HIV exposure. In: Biotechnology Advances. 1997 ; Vol. 15, No. 3-4. pp. 703.
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